Radioimmunoassay of fragment E‐related neoantigen

validation studies and clinical application

James Chen, Wahid Hanna, Teresa K. Williams, Stephen Krauss

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Summary. Measurement of fibrinogen‐fibrin degradation products (FDP) levels in plasma may provide a direct index of plasmin action, and increased levels of FDP would indicate coagulopathy. We have established an E‐neoantigen radioimmunoassay (Eneo RIA) that can determine normal and pathological plasma levels of E‐related FDP. The assay employs rabbit antiserum produced against fragment E derived from a plasmin digest of fibrinogen and subsequently absorbed with fibrinogen. The absorbed antiserum contains antibodies which are equally reactive with fibrinogen derived E (Fg‐E) and fibrin derived E (Fb‐E) but not with fibrinogen at 1 mg/ml. The Eneo RIA was validated by assay parallelism and by recovery experiments. Plasma Eneo immunoreactivities in 14 normals were 4–22 ng/ml (mean 12.7 ng/ml). Plasma Eneo levels in 23 of 24 patients with neoplastic and haematological diseases were elevated above normal (range 27–2027 ng/ml). Unusually high Eneo values were observed with three patients whose diseases were complicated by either disseminated intravascular coagulation (DIC) or deep vein thrombosis. After heparin therapy, the Eneo level of a patient with chronic DIC declined. A pathological plasma was eluted from a Sephadex G‐200 column and Eneo immunoreactivity was determined on the eluates. The gel filtration pattern of Eneo indicates that E‐related FDP is a family of plasmic fragments derived from crosslinked fibrin.

Original languageEnglish (US)
Pages (from-to)133-144
Number of pages12
JournalBritish Journal of Haematology
Volume57
Issue number1
DOIs
StatePublished - Jan 1 1984
Externally publishedYes

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Validation Studies
Radioimmunoassay
Fibrinogen
Disseminated Intravascular Coagulation
Fibrinolysin
Fibrin
Immune Sera
Hematologic Diseases
Venous Thrombosis
Gel Chromatography
Heparin
Reference Values
Rabbits
Antibodies

All Science Journal Classification (ASJC) codes

  • Hematology

Cite this

Radioimmunoassay of fragment E‐related neoantigen : validation studies and clinical application. / Chen, James; Hanna, Wahid; Williams, Teresa K.; Krauss, Stephen.

In: British Journal of Haematology, Vol. 57, No. 1, 01.01.1984, p. 133-144.

Research output: Contribution to journalArticle

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