Rapid diagnosis of ocular herpes simplex infections

Penny Asbell, M. A. Torres, T. Kamenar, E. J. Bottone

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background-The Surecell herpes (HSV) test kit is a test for detecting the presence of herpes simplex viral antigen by,means of a monoclonal antibody based immunoassay. The test has proved to be highly sensitive and specific in diagnosing genital, oral, and dermatological herpes infections. Methods-In this study, samples from patients with ocular keratitis were evaluated by tissue cultures and the Surecell test. The eyes of New Zealand rabbits were then inoculated with HSV type 1 acute keratitis, acute Staphylococcus keratitis, acute Pseudomonas keratitis, and HSV type 1 postkeratitis (healed corneas). Tear film samples collected from each eye with a cotton swab were evaluated by routine culture (A-549 monolayers) and by the Surecell test with and without prior placement of the swab in Hank's medium. Results-The Surecell system had a 70% sensitivity and a 100% specificity in the detection of HSV antigen in ocular infections, and was shown to be a quick, efficient, and accurate method of testing for HSV antigen in humans. Conclusion-These results from humans and rabbits indicate that the Surecell test, which requires no special equipment, can be a useful in office adjunct in the clinical diagnosis of ocular herpes simplex.

Original languageEnglish (US)
Pages (from-to)473-475
Number of pages3
JournalBritish Journal of Ophthalmology
Volume79
Issue number5
DOIs
StatePublished - Jan 1 1995

Fingerprint

Herpetic Keratitis
Keratitis
Human Herpesvirus 1
Infection
Rabbits
Eye Infections
Antigens
Herpes Simplex
Viral Antigens
Pseudomonas
Tears
Staphylococcus
Immunoassay
Cornea
Monoclonal Antibodies
Equipment and Supplies

All Science Journal Classification (ASJC) codes

  • Critical Care and Intensive Care Medicine
  • Emergency Medicine
  • Ophthalmology

Cite this

Rapid diagnosis of ocular herpes simplex infections. / Asbell, Penny; Torres, M. A.; Kamenar, T.; Bottone, E. J.

In: British Journal of Ophthalmology, Vol. 79, No. 5, 01.01.1995, p. 473-475.

Research output: Contribution to journalArticle

Asbell, Penny ; Torres, M. A. ; Kamenar, T. ; Bottone, E. J. / Rapid diagnosis of ocular herpes simplex infections. In: British Journal of Ophthalmology. 1995 ; Vol. 79, No. 5. pp. 473-475.
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