Rapid fabrication of poly(dimethylsiloxane)-based microchip capillary electrophoresis devices using CO2 laser ablation

Barbara A. Fogarty, Kathleen E. Heppert, Theodore Cory, Kalonie R. Hulbutta, R. Scott Martin, Susan M. Lunte

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The use of CO2 laser ablation for the patterning of capillary electrophoresis (CE) microchannels in poly(dimethylsiloxane) (PDMS) is described. Low-cost polymer devices were produced using a relatively inexpensive CO2 laser system that facilitated rapid patterning and ablation of microchannels. Device designs were created using a commercially available software package. The effects of PDMS thickness, laser focusing, power, and speed on the resulting channel dimensions were investigated. Using optimized settings, the smallest channels that could be produced averaged 33 μm in depth (11.1% RSD, N = 6) and 110 μm in width (5.7% RSD, N = 6). The use of a PDMS substrate allowed reversible sealing of microchip components at room temperature without the need for cleanroom facilities. Using a layer of pre-cured polymer, devices were designed, ablated, and assembled within minutes. The final devices were used for microchip CE separation and detection of the fluorescently labeled neurotransmitters aspartate and glutamate.

Original languageEnglish (US)
Pages (from-to)924-930
Number of pages7
JournalAnalyst
Volume130
Issue number6
DOIs
StatePublished - Jun 1 2005
Externally publishedYes

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Microchip Electrophoresis
Capillary electrophoresis
Gas Lasers
Capillary Electrophoresis
Laser Therapy
Laser ablation
Polydimethylsiloxane
Microchannels
ablation
electrokinesis
laser
Fabrication
Equipment and Supplies
Polymers
polymer
Equipment Design
sealing
Aspartic Acid
Neurotransmitter Agents
Lasers

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Rapid fabrication of poly(dimethylsiloxane)-based microchip capillary electrophoresis devices using CO2 laser ablation. / Fogarty, Barbara A.; Heppert, Kathleen E.; Cory, Theodore; Hulbutta, Kalonie R.; Martin, R. Scott; Lunte, Susan M.

In: Analyst, Vol. 130, No. 6, 01.06.2005, p. 924-930.

Research output: Contribution to journalArticle

Fogarty, Barbara A. ; Heppert, Kathleen E. ; Cory, Theodore ; Hulbutta, Kalonie R. ; Martin, R. Scott ; Lunte, Susan M. / Rapid fabrication of poly(dimethylsiloxane)-based microchip capillary electrophoresis devices using CO2 laser ablation. In: Analyst. 2005 ; Vol. 130, No. 6. pp. 924-930.
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