Rat monoclonal antibodies to mouse lung components for analysis of fibrosis

Stephen Kennel, J. A. Hotchkiss, Marie C. Rorvik, David P. Allison, Linda J. Foote

Research output: Contribution to journalArticle

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Abstract

Six rat monoclonal antibodies to mouse lung membrane fraction have been characterized. Each has unique binding properties and can be used to stain particular lung components in paraffin sections. One antibody, 133-13A, recognizes a 100-kDa glycoprotein on lung tumor cells, but stains only macrophage-like cells in normal or fibrotic lung sections as determined by immunogold electron microscopy. The monoclonal antibody 273-34A binds to a 112-kDa protein on the surface of normal mouse lung fibroblasts. Immunogold electron microscopy demonstrates antibody binding to capillary endothelial cells, but not to fibroblasts, Type I, or Type II cells. Staining of fibrotic sections with MoAb 273-34A is dramatically enhanced over staining of normal lung. A third antibody, 370-8A, gives a general staining pattern throughout normal lung that is intensified in fibrotic lung. Another MoAb 328-41A mediates intense nuclear fluorescence of lung tumor cells and cells in lung sections. It binds to 14-and 17-kDa proteins that may be high mobility group (HMG) nuclear proteins. Enzyme inhibition studies and immunofluorescence staining patterns on normal lung indicate that elastin may be the target of MoAb 328-35B. MoAb 327-5B binds to normal mouse lung fibroblasts and red blood cell membranes. These last three MoAbs stain macrophages in fibrotic lung, but give a general pattern of light epithelial cell stain in normal lung sections indicating macrophage engulfment of the normal cell antigen during fibrosis. These antibodies should be useful in identifying cell types and molecular mechanisms involved in early stages of fibrosis induced by different chemical insults.

Original languageEnglish (US)
Pages (from-to)110-124
Number of pages15
JournalExperimental and Molecular Pathology
Volume47
Issue number1
DOIs
StatePublished - Jan 1 1987
Externally publishedYes

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Rats
Macrophages
Fibrosis
Coloring Agents
Fibroblasts
Monoclonal Antibodies
Lung
Antibodies
Electron microscopy
Tumors
Cells
Enzyme inhibition
Elastin
Endothelial cells
Cell membranes
Nuclear Proteins
Paraffin
Glycoproteins
Proteins
Blood

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Rat monoclonal antibodies to mouse lung components for analysis of fibrosis. / Kennel, Stephen; Hotchkiss, J. A.; Rorvik, Marie C.; Allison, David P.; Foote, Linda J.

In: Experimental and Molecular Pathology, Vol. 47, No. 1, 01.01.1987, p. 110-124.

Research output: Contribution to journalArticle

Kennel, Stephen ; Hotchkiss, J. A. ; Rorvik, Marie C. ; Allison, David P. ; Foote, Linda J. / Rat monoclonal antibodies to mouse lung components for analysis of fibrosis. In: Experimental and Molecular Pathology. 1987 ; Vol. 47, No. 1. pp. 110-124.
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