Real time monitoring of endothelial cell actin filament disruption by cytochalasin D using a cellular impedance biosensor

V. Nandakumar, A. E. English, A. B. Moy, M. Mahfouz, R. Ward, K. Kruse, S. Kirkpatrick, Mitchell Goldman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Using a novel cellular impedance biosensor and confocal microscopy, this study has examined the dynamic and steady state cellular impedance response of porcine pulmonary endothelial cells to varying doses of cytochalasin D. Endothelial cell monolayer impedances were obtained using an array of gold microelectrodes coated with fibronectin to facilitate endothelial cell adhesion. Impedance measurements were acquired at 5.6 kHz by phase sensitive detection from a lock-in amplifier. The electrically measured cytochalasin D dose dependant actin disruption was successfully correlated with actin stained confocal microscopy images quantified using image-processing techniques. Based on this study, the cellular kinetic response to cytochalasin D increased systematically with the dose and saturated at a critical concentration. The real time quantification of pharmacological agents that target specific elements of the cytoskeleton using electrical impedance measurements therefore has a number of important applications in understanding the dynamic and steady state response of endothelial cells to toxins and drug induced cellular cytoskeletal micromechanics.

Original languageEnglish (US)
Title of host publication2004 2nd IEEE/EMBS International Summer School on Medical Devices and Biosensors, ISSS-MDBS 2004
Pages85-86
Number of pages2
StatePublished - Dec 1 2004
Event2004 2nd IEEE/EMBS International Summer School on Medical Devices and Biosensors, ISSS-MDBS 2004 - Hong Kong, China
Duration: Jun 26 2004Jul 2 2004

Other

Other2004 2nd IEEE/EMBS International Summer School on Medical Devices and Biosensors, ISSS-MDBS 2004
CountryChina
CityHong Kong
Period6/26/047/2/04

Fingerprint

Cytochalasin D
Endothelial cells
Biosensing Techniques
Electric Impedance
Actin Cytoskeleton
Biosensors
Actins
Endothelial Cells
Monitoring
Confocal microscopy
Confocal Microscopy
Acoustic impedance
Micromechanics
Microelectrodes
Cell adhesion
Fibronectins
Chemical elements
Gold
Somatostatin-Secreting Cells
Monolayers

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering
  • Materials Science(all)
  • Medicine(all)

Cite this

Nandakumar, V., English, A. E., Moy, A. B., Mahfouz, M., Ward, R., Kruse, K., ... Goldman, M. (2004). Real time monitoring of endothelial cell actin filament disruption by cytochalasin D using a cellular impedance biosensor. In 2004 2nd IEEE/EMBS International Summer School on Medical Devices and Biosensors, ISSS-MDBS 2004 (pp. 85-86). [1689567]

Real time monitoring of endothelial cell actin filament disruption by cytochalasin D using a cellular impedance biosensor. / Nandakumar, V.; English, A. E.; Moy, A. B.; Mahfouz, M.; Ward, R.; Kruse, K.; Kirkpatrick, S.; Goldman, Mitchell.

2004 2nd IEEE/EMBS International Summer School on Medical Devices and Biosensors, ISSS-MDBS 2004. 2004. p. 85-86 1689567.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nandakumar, V, English, AE, Moy, AB, Mahfouz, M, Ward, R, Kruse, K, Kirkpatrick, S & Goldman, M 2004, Real time monitoring of endothelial cell actin filament disruption by cytochalasin D using a cellular impedance biosensor. in 2004 2nd IEEE/EMBS International Summer School on Medical Devices and Biosensors, ISSS-MDBS 2004., 1689567, pp. 85-86, 2004 2nd IEEE/EMBS International Summer School on Medical Devices and Biosensors, ISSS-MDBS 2004, Hong Kong, China, 6/26/04.
Nandakumar V, English AE, Moy AB, Mahfouz M, Ward R, Kruse K et al. Real time monitoring of endothelial cell actin filament disruption by cytochalasin D using a cellular impedance biosensor. In 2004 2nd IEEE/EMBS International Summer School on Medical Devices and Biosensors, ISSS-MDBS 2004. 2004. p. 85-86. 1689567
Nandakumar, V. ; English, A. E. ; Moy, A. B. ; Mahfouz, M. ; Ward, R. ; Kruse, K. ; Kirkpatrick, S. ; Goldman, Mitchell. / Real time monitoring of endothelial cell actin filament disruption by cytochalasin D using a cellular impedance biosensor. 2004 2nd IEEE/EMBS International Summer School on Medical Devices and Biosensors, ISSS-MDBS 2004. 2004. pp. 85-86
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N2 - Using a novel cellular impedance biosensor and confocal microscopy, this study has examined the dynamic and steady state cellular impedance response of porcine pulmonary endothelial cells to varying doses of cytochalasin D. Endothelial cell monolayer impedances were obtained using an array of gold microelectrodes coated with fibronectin to facilitate endothelial cell adhesion. Impedance measurements were acquired at 5.6 kHz by phase sensitive detection from a lock-in amplifier. The electrically measured cytochalasin D dose dependant actin disruption was successfully correlated with actin stained confocal microscopy images quantified using image-processing techniques. Based on this study, the cellular kinetic response to cytochalasin D increased systematically with the dose and saturated at a critical concentration. The real time quantification of pharmacological agents that target specific elements of the cytoskeleton using electrical impedance measurements therefore has a number of important applications in understanding the dynamic and steady state response of endothelial cells to toxins and drug induced cellular cytoskeletal micromechanics.

AB - Using a novel cellular impedance biosensor and confocal microscopy, this study has examined the dynamic and steady state cellular impedance response of porcine pulmonary endothelial cells to varying doses of cytochalasin D. Endothelial cell monolayer impedances were obtained using an array of gold microelectrodes coated with fibronectin to facilitate endothelial cell adhesion. Impedance measurements were acquired at 5.6 kHz by phase sensitive detection from a lock-in amplifier. The electrically measured cytochalasin D dose dependant actin disruption was successfully correlated with actin stained confocal microscopy images quantified using image-processing techniques. Based on this study, the cellular kinetic response to cytochalasin D increased systematically with the dose and saturated at a critical concentration. The real time quantification of pharmacological agents that target specific elements of the cytoskeleton using electrical impedance measurements therefore has a number of important applications in understanding the dynamic and steady state response of endothelial cells to toxins and drug induced cellular cytoskeletal micromechanics.

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