Receptor-binding specificity of the human parainfluenza virus type 1 hemagglutinin-neuraminidase glycoprotein

Irina V. Alymova, Allen Portner, Vasiliy P. Mishin, Jonathan Mccullers, Pamela Freiden, Garry L. Taylor

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The hemagglutinin-neuraminidase (HN) glycoprotein is utilized by human parainfluenza viruses for binding to the host cell. By the use of glycan array assays, we demonstrate that, in addition to the first catalytic-binding site, the HN of human parainfluenza virus type 1 has a second site for binding covered by N-linked glycan. Our data suggest that attachment of the first site to sialic acid (SA)-linked receptors triggers exposure of the second site. We found that both sites bind to α2-3-linked SAs with a preference for a sialyl-Lewis x motif. Binding to α2-3-linked SAs with a sulfated sialyl-Lewis motif as well as to α2-8-linked SAs was unique for the second binding site. Neither site recognizes α2-6-linked oligosaccharides.

Original languageEnglish (US)
Pages (from-to)174-180
Number of pages7
JournalGlycobiology
Volume22
Issue number2
DOIs
StatePublished - Feb 1 2012
Externally publishedYes

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Human parainfluenza virus 1
HN Protein
Viruses
Binding Sites
Polysaccharides
Virus Attachment
Paramyxoviridae Infections
Hemagglutinins
Neuraminidase
N-Acetylneuraminic Acid
Oligosaccharides
Assays
Catalytic Domain

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Receptor-binding specificity of the human parainfluenza virus type 1 hemagglutinin-neuraminidase glycoprotein. / Alymova, Irina V.; Portner, Allen; Mishin, Vasiliy P.; Mccullers, Jonathan; Freiden, Pamela; Taylor, Garry L.

In: Glycobiology, Vol. 22, No. 2, 01.02.2012, p. 174-180.

Research output: Contribution to journalArticle

Alymova, Irina V. ; Portner, Allen ; Mishin, Vasiliy P. ; Mccullers, Jonathan ; Freiden, Pamela ; Taylor, Garry L. / Receptor-binding specificity of the human parainfluenza virus type 1 hemagglutinin-neuraminidase glycoprotein. In: Glycobiology. 2012 ; Vol. 22, No. 2. pp. 174-180.
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