Receptors for advanced glycation end-products (AGE) - Expression by endothelial cells in non-diabetic uraemic patients

J. Greten, I. Kreis, K. Wiesel, E. Stier, A. M. Schmidt, David Stern, E. Ritz, R. Waldherr, P. P. Nawroth

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Background. Cellular actions of advanced glycation end-products (AGE) are mediated by a receptor for AGE (RAGE), a novel integral membrane protein. Immunohistochemical studies show only low-level RAGE antigen expression in endothelial cells. Design. It was the purpose of the study to compare expression of RAGE antigen by endothelial cells in non-diabetic uraemic patients (n = 8) with non-uraemic controls (n = 11). Samples of arterial tissue were obtained at the time of renal transplantation (in uraemic patients) and abdominal surgery (in controls). RAGE antigen was visualized using guinea-pig anti-RAGE IgG and PAP technique. Results. Marked staining for RAGE was noted in endothelial cells, both arterial endothelium and endothelium of vasa vasorum of normoglycaemic uraemic patients, but was not demonstrable in endothelial cells of large arteries and only faintly expressed in vasa vasorum of non-uraemic individuals. Conclusion. Normal endothelial cells do not constitually express RAGE antigen: in contrast it is expressed by arterial and capillary endothelial cells of uraemic patients. The observation is of note in view of the putative role of AGE of causing atherosclerotic and non-atherosclerotic vascular lesions.

Original languageEnglish (US)
Pages (from-to)789-790
Number of pages2
JournalNephrology Dialysis Transplantation
Volume11
Issue number5
StatePublished - May 1 1996
Externally publishedYes

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Endothelial Cells
Antigen Receptors
Vasa Vasorum
Advanced Glycosylation End Products
Endothelium
IgG Receptors
Advanced Glycosylation End Product-Specific Receptor
Kidney Transplantation
Blood Vessels
Guinea Pigs
Membrane Proteins
Arteries
Observation
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Nephrology
  • Transplantation

Cite this

Receptors for advanced glycation end-products (AGE) - Expression by endothelial cells in non-diabetic uraemic patients. / Greten, J.; Kreis, I.; Wiesel, K.; Stier, E.; Schmidt, A. M.; Stern, David; Ritz, E.; Waldherr, R.; Nawroth, P. P.

In: Nephrology Dialysis Transplantation, Vol. 11, No. 5, 01.05.1996, p. 789-790.

Research output: Contribution to journalArticle

Greten, J, Kreis, I, Wiesel, K, Stier, E, Schmidt, AM, Stern, D, Ritz, E, Waldherr, R & Nawroth, PP 1996, 'Receptors for advanced glycation end-products (AGE) - Expression by endothelial cells in non-diabetic uraemic patients', Nephrology Dialysis Transplantation, vol. 11, no. 5, pp. 789-790.
Greten, J. ; Kreis, I. ; Wiesel, K. ; Stier, E. ; Schmidt, A. M. ; Stern, David ; Ritz, E. ; Waldherr, R. ; Nawroth, P. P. / Receptors for advanced glycation end-products (AGE) - Expression by endothelial cells in non-diabetic uraemic patients. In: Nephrology Dialysis Transplantation. 1996 ; Vol. 11, No. 5. pp. 789-790.
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