Recognition of structurally diverse substrates by type II 3-hydroxyacyl-CoA dehydrogenase (HADH II)/amyloid-β binding alcohol dehydrogenase (ABAD)

A. J. Powell, J. A. Read, M. J. Banfield, F. Gunn-Moore, S. D. Yan, J. Lustbader, A. R. Stern, David Stern, R. L. Brady

Research output: Contribution to journalArticle

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Abstract

Human type II hydroxyacyl-CoA dehydrogenase/amyloid-β binding alcohol dehydrogenase (HADH II/ABAD) is an oxidoreductase whose salient features include broad substrate specificity, encompassing 3-hydroxyacyl-CoA derivatives, hydroxysteroids, alcohols and β-hydroxybutyrate, and the capacity to bind amyloid-β peptide, leading to propagation of amyloid-induced cell stress. In this study, we examine the structure and enzymatic activity of the homologous rat HADH II/ABAD enzyme. We report the crystal structure of rat HADH II/ABAD as a binary complex with its NADH cofactor to 2.0 Å resolution, as a ternary complex with NAD + and 3-ketobutyrate (acetoacetate) to 1.4 Å resolution, and as a ternary complex with NADH and 17β-estradiol to 1.7 Å resolution. This first crystal structure of an HADH II confirms these enzymes are closely related to the short-chain hydroxysteroid dehydrogenases and differ substantially from the classic, type I 3-hydroxyacyl-CoA dehydrogenases. Binding of the ketobutyrate substrate is accompanied by closure of the active site specificity loop, whereas the steroid substrate does not appear to require closure for binding. Despite the different chemical nature of the two bound substrates, the presentation of chemical groups within the active site of each complex is remarkably similar, allowing a general mechanism for catalytic activity to be proposed. There is a characteristic extension to the active site that is likely to accommodate the CoA moiety of 3-hydroxyacyl-CoA substrates. Rat HADH II/ABAD also binds amyloid-β (1-40) peptide with a K(D) of 21 nM, which is similar to the interaction exhibited between this peptide and human HADH II/ABAD. These studies provide the first structural insights into HADH II/ABAD interaction with its substrates, and indicate the relevance of the rodent enzyme and associated rodent models for analysis of HADH II/ABAD's physiologic and pathophysiologic properties. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)311-327
Number of pages17
JournalJournal of Molecular Biology
Volume303
Issue number2
DOIs
StatePublished - Oct 20 2000
Externally publishedYes

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3-Hydroxyacyl-CoA Dehydrogenase
Alcohol Dehydrogenase
Amyloid
Coenzyme A
NAD
Catalytic Domain
Peptides
Rodentia
3-Hydroxyacyl CoA Dehydrogenases
Oxidoreductases
Enzymes
Hydroxysteroids
Hydroxybutyrates
Hydroxysteroid Dehydrogenases
Substrate Specificity
Estradiol

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Cite this

Recognition of structurally diverse substrates by type II 3-hydroxyacyl-CoA dehydrogenase (HADH II)/amyloid-β binding alcohol dehydrogenase (ABAD). / Powell, A. J.; Read, J. A.; Banfield, M. J.; Gunn-Moore, F.; Yan, S. D.; Lustbader, J.; Stern, A. R.; Stern, David; Brady, R. L.

In: Journal of Molecular Biology, Vol. 303, No. 2, 20.10.2000, p. 311-327.

Research output: Contribution to journalArticle

Powell, A. J. ; Read, J. A. ; Banfield, M. J. ; Gunn-Moore, F. ; Yan, S. D. ; Lustbader, J. ; Stern, A. R. ; Stern, David ; Brady, R. L. / Recognition of structurally diverse substrates by type II 3-hydroxyacyl-CoA dehydrogenase (HADH II)/amyloid-β binding alcohol dehydrogenase (ABAD). In: Journal of Molecular Biology. 2000 ; Vol. 303, No. 2. pp. 311-327.
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AU - Yan, S. D.

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