Recombinant chimeric borrelia proteins for diagnosis of Lyme disease

Maria Gomes-Solecki, John J. Dunn, Benjamin J. Luft, Jonathan Castillo, Daniel E. Dykhuizen, Xiaohua Yang, John D. Glass, Raymond J. Dattwyler

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Current serologic Lyme disease tests use whole borrelia cells as the source of antigen. These assays are difficult to standardize and to optimize for sensitivity and specificity. To help solve these problems, we constructed a library of recombinant chimeric proteins composed of portions of key antigens of Borrelia burgdorferi. These proteins were then used to develop an enzyme-linked immunosorbent assay. We compared our assay with the most sensitive of three whole-cell borrelia assays. We found that the recombinant assay could detect antibodies significantly better from early Lyme disease sera (P < 0.05), and bad the same sensitivity for late Lyme disease sera, as the most sensitive whole-cell borrelia assay. On potentially cross-reactive sera, the recombinant assay was more specific, but not significantly so, than the best whole-cell borrelia assay. Optimization of the recombinant assay offers the potential for a significant improvement in both sensitivity and specificity.

Original languageEnglish (US)
Pages (from-to)2530-2535
Number of pages6
JournalJournal of Clinical Microbiology
Volume38
Issue number7
StatePublished - 2000
Externally publishedYes

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Recombinant Fusion Proteins
Borrelia
Lyme Disease
Serum
Antigens
Sensitivity and Specificity
Borrelia burgdorferi
Enzyme-Linked Immunosorbent Assay
Antibodies
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Gomes-Solecki, M., Dunn, J. J., Luft, B. J., Castillo, J., Dykhuizen, D. E., Yang, X., ... Dattwyler, R. J. (2000). Recombinant chimeric borrelia proteins for diagnosis of Lyme disease. Journal of Clinical Microbiology, 38(7), 2530-2535.

Recombinant chimeric borrelia proteins for diagnosis of Lyme disease. / Gomes-Solecki, Maria; Dunn, John J.; Luft, Benjamin J.; Castillo, Jonathan; Dykhuizen, Daniel E.; Yang, Xiaohua; Glass, John D.; Dattwyler, Raymond J.

In: Journal of Clinical Microbiology, Vol. 38, No. 7, 2000, p. 2530-2535.

Research output: Contribution to journalArticle

Gomes-Solecki, M, Dunn, JJ, Luft, BJ, Castillo, J, Dykhuizen, DE, Yang, X, Glass, JD & Dattwyler, RJ 2000, 'Recombinant chimeric borrelia proteins for diagnosis of Lyme disease', Journal of Clinical Microbiology, vol. 38, no. 7, pp. 2530-2535.
Gomes-Solecki M, Dunn JJ, Luft BJ, Castillo J, Dykhuizen DE, Yang X et al. Recombinant chimeric borrelia proteins for diagnosis of Lyme disease. Journal of Clinical Microbiology. 2000;38(7):2530-2535.
Gomes-Solecki, Maria ; Dunn, John J. ; Luft, Benjamin J. ; Castillo, Jonathan ; Dykhuizen, Daniel E. ; Yang, Xiaohua ; Glass, John D. ; Dattwyler, Raymond J. / Recombinant chimeric borrelia proteins for diagnosis of Lyme disease. In: Journal of Clinical Microbiology. 2000 ; Vol. 38, No. 7. pp. 2530-2535.
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