Recombinant tetravalent group A streptococcal M protein vaccine

James Dale, E. Y. Chiang, J. W. Lederer

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Previous studies have shown that the amino-terminal regions of group A streptococcal M proteins contain primarily protective (opsonic) epitopes and not tissue-cross-reactive epitopes. Limited primary structures from multiple serotypes of M protein containing only protective epitopes could potentially be linked together to form a broadly protective vaccine. The present studies were undertaken to determine the protective immunogenicity of a recombinant, multivalent hybrid molecule containing amino-terminal subunits of types 24, 5, 6, and 19 M proteins. Polymerase chain reaction primers were designed to amplify emm gene fragments ranging from 35 to 113 codons. The PCR products were ligated in tandem and inserted into pKK223-3. The tetravalent M protein that was purified from extracts of Escherichia coli migrated as a single band on SDS-PAGE with an apparent m.w. of 31 kDa. In immunoblot analyses, the hybrid protein reacted with serotype-specific antisera indicating that it contained all four M protein subunits. Rabbits immunized with the purified tetravalent M protein developed significant antibody levels against all four serotypes of native M proteins represented in the hybrid protein. None of the antisera cross-reacted with human tissues. The immune sera also opsonized all four serotypes of group A streptococci. Our data show that a hybrid protein containing subunits from multiple M proteins can evoke broadly protective immune responses without tissue-cross-reactive antibodies.

Original languageEnglish (US)
Pages (from-to)2188-2194
Number of pages7
JournalJournal of Immunology
Volume151
Issue number4
StatePublished - Jan 1 1993

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Vaccines
Proteins
Immune Sera
Epitopes
Protein Subunits
Polymerase Chain Reaction
streptococcal M protein
Antibodies
Streptococcus
Codon
Polyacrylamide Gel Electrophoresis
Escherichia coli
Rabbits
Serogroup
Genes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Dale, J., Chiang, E. Y., & Lederer, J. W. (1993). Recombinant tetravalent group A streptococcal M protein vaccine. Journal of Immunology, 151(4), 2188-2194.

Recombinant tetravalent group A streptococcal M protein vaccine. / Dale, James; Chiang, E. Y.; Lederer, J. W.

In: Journal of Immunology, Vol. 151, No. 4, 01.01.1993, p. 2188-2194.

Research output: Contribution to journalArticle

Dale, J, Chiang, EY & Lederer, JW 1993, 'Recombinant tetravalent group A streptococcal M protein vaccine', Journal of Immunology, vol. 151, no. 4, pp. 2188-2194.
Dale, James ; Chiang, E. Y. ; Lederer, J. W. / Recombinant tetravalent group A streptococcal M protein vaccine. In: Journal of Immunology. 1993 ; Vol. 151, No. 4. pp. 2188-2194.
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