Recovery of multipotent progenitors from the peripheral blood of patients requiring extracorporeal membrane oxygenation support

Kim Chi T. Bui, Dinithi Senadheera, Xingchao Wang, Benjamin Hendrickson, Philippe Friedlich, Carolyn Lutzko

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Rationale: Studies have demonstrated that bone marrow-derived cells can be recruited to injured lungs through an unknown mechanism. We hypothesize that marrow progenitors are mobilized into the circulation of patients with cardiac and/or respiratory failure, and may then traffic to and incorporate into the sites of tissue injury. Objectives: To determine whether progenitor populations are increased in the blood of patients with severe acute cardiorespiratory failure placed on extracorporeal membrane oxygenation (ECMO). Methods: Mononuclear cells from ECMO, umbilical cord, and control blood samples were evaluated in colony-forming assays for hematopoietic, mesenchymal, and epithelial cells. Progenitors were identified by proliferative and differentiative capacities, and confirmed by the expression of lineage-specific markers. Measurements and Main Results: Significantly higher levels of hematopoietic progenitors were observed inECMO(n = 41) samples than neonatal intensive care unit (n = 16) or pediatric intensive care unit controls (n = 14). Hematopoietic progenitor mobilization increased with time on ECMO support. Mesenchymal progenitors (MSC) were recovered from 18/58 ECMO samples with rapid sample processing (< 4 h) critical to their recovery. MSC were not recovered from normal controls. ECMO-derivedMSC had osteogenic, chondrogenic, andadipogenicdifferentiationpotential. The recovery ofMSCdid not influence survival outcome (61%). Epithelial progenitors were observed in eight ECMO samples but not in control samples. Their presence was associated with a lower survival trend (38%). Conclusions:Hematopoietic,mesenchymal,andepithelial progenitors were mobilized into the circulation of patients on ECMO. This may reflect a response to severe cardiopulmonary injury, blood-foreign surface interactions with the ECMO circuit, and/or hemodilution.

Original languageEnglish (US)
Pages (from-to)226-237
Number of pages12
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume181
Issue number3
DOIs
StatePublished - Feb 1 2010
Externally publishedYes

Fingerprint

Extracorporeal Membrane Oxygenation
Pediatric Intensive Care Units
Hemodilution
Survival
Neonatal Intensive Care Units
Wounds and Injuries
Fetal Blood
Bone Marrow Cells
Respiratory Insufficiency
Heart Failure
Bone Marrow
Epithelial Cells
Cell Membrane
Lung

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Recovery of multipotent progenitors from the peripheral blood of patients requiring extracorporeal membrane oxygenation support. / Bui, Kim Chi T.; Senadheera, Dinithi; Wang, Xingchao; Hendrickson, Benjamin; Friedlich, Philippe; Lutzko, Carolyn.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 181, No. 3, 01.02.2010, p. 226-237.

Research output: Contribution to journalArticle

Bui, Kim Chi T. ; Senadheera, Dinithi ; Wang, Xingchao ; Hendrickson, Benjamin ; Friedlich, Philippe ; Lutzko, Carolyn. / Recovery of multipotent progenitors from the peripheral blood of patients requiring extracorporeal membrane oxygenation support. In: American Journal of Respiratory and Critical Care Medicine. 2010 ; Vol. 181, No. 3. pp. 226-237.
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