Regional brain blood flow in mouse

Quantitative measurement using a single-pass radio-tracer method and a mathematical algorithm

K. Xu, K. Radhakrishnan, A. Serhal, F. Allen, J. C. LaManna, Michelle Puchowicz

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

We1 have developed a reliable experimental method for measuring local regional cerebral blood flows in anesthetized mice. This method is an extension of the well-established single-pass dual-label indicator method for simultaneously measuring blood flow and glucose influx in rat brains. C57BL6J mice (n = 10) were anesthetized and regional blood flows (ml/min/g) were measured using the radio-tracer method. To test the sensitivity of this method we used a mathematical algorithm to predict the blood flows and compared the two sets of results.Measured regional blood flows between 0.7 and 1.7 ml/min/g were similar to those we have previously reported in the rat. The predicted blood flows using an assumed linearly increasing arterial tracer concentration-versus- time profile (that is, a ramp) were similar to the valuesmeasured in the physiological experiments (R2 0.99; slope 0.91). Thus,measurements of local regional cerebral blood flow in anesthetized mice using a single-pass radio-tracer method appear to be reliable.

Original languageEnglish (US)
Title of host publicationOxygen Transport to Tissue XXXII
PublisherSpringer New York LLC
Pages255-260
Number of pages6
ISBN (Print)9781441977557
DOIs
StatePublished - Jan 1 2011

Publication series

NameAdvances in Experimental Medicine and Biology
Volume701
ISSN (Print)0065-2598

Fingerprint

Regional Blood Flow
Radio
Brain
Blood
Cerebrovascular Circulation
Rats
Architectural Accessibility
Blood Glucose
Labels
Glucose

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Xu, K., Radhakrishnan, K., Serhal, A., Allen, F., LaManna, J. C., & Puchowicz, M. (2011). Regional brain blood flow in mouse: Quantitative measurement using a single-pass radio-tracer method and a mathematical algorithm. In Oxygen Transport to Tissue XXXII (pp. 255-260). (Advances in Experimental Medicine and Biology; Vol. 701). Springer New York LLC. https://doi.org/10.1007/978-1-4419-7756-4_34

Regional brain blood flow in mouse : Quantitative measurement using a single-pass radio-tracer method and a mathematical algorithm. / Xu, K.; Radhakrishnan, K.; Serhal, A.; Allen, F.; LaManna, J. C.; Puchowicz, Michelle.

Oxygen Transport to Tissue XXXII. Springer New York LLC, 2011. p. 255-260 (Advances in Experimental Medicine and Biology; Vol. 701).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Xu, K, Radhakrishnan, K, Serhal, A, Allen, F, LaManna, JC & Puchowicz, M 2011, Regional brain blood flow in mouse: Quantitative measurement using a single-pass radio-tracer method and a mathematical algorithm. in Oxygen Transport to Tissue XXXII. Advances in Experimental Medicine and Biology, vol. 701, Springer New York LLC, pp. 255-260. https://doi.org/10.1007/978-1-4419-7756-4_34
Xu K, Radhakrishnan K, Serhal A, Allen F, LaManna JC, Puchowicz M. Regional brain blood flow in mouse: Quantitative measurement using a single-pass radio-tracer method and a mathematical algorithm. In Oxygen Transport to Tissue XXXII. Springer New York LLC. 2011. p. 255-260. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-1-4419-7756-4_34
Xu, K. ; Radhakrishnan, K. ; Serhal, A. ; Allen, F. ; LaManna, J. C. ; Puchowicz, Michelle. / Regional brain blood flow in mouse : Quantitative measurement using a single-pass radio-tracer method and a mathematical algorithm. Oxygen Transport to Tissue XXXII. Springer New York LLC, 2011. pp. 255-260 (Advances in Experimental Medicine and Biology).
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