Regulation of allantoate transport in wild-type and mutant strains of Saccharomyces cerevisiae

V. T. Chisholm, H. Z. Lea, R. Rai, Terrance Cooper

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Accumulation of intracellular allantoin and allantoate is mediated by two distinct active transport systems in Saccharomyces cerevisiae. Allantoin transport (DAL4 gene) is inducible, while allantoate uptake is constitutive (it occurs at full levels in the absence of any allantoate-related compounds from the culture medium). Both systems appear to be sensitive to nitrogen catabolite repression, feedback inhibition, and trans-inhibition. Mutants (dal5) that lack allantoate transport have been isolated. These strains also exhibit a 60% loss of allantoin transport capability. Conversely, dal4 mutants previously described are unable to transport allantoin and exhibit a 50% loss of allantoate transport. We interpret the pleiotropic behavior of the dal4 and dal5 mutations as deriving from a functional interaction between elements of the two transport systems.

Original languageEnglish (US)
Pages (from-to)1684-1690
Number of pages7
JournalJournal of bacteriology
Volume169
Issue number4
DOIs
StatePublished - Jan 1 1987

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Allantoin
Saccharomyces cerevisiae
Catabolite Repression
Active Biological Transport
Culture Media
Nitrogen
Mutation
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Regulation of allantoate transport in wild-type and mutant strains of Saccharomyces cerevisiae. / Chisholm, V. T.; Lea, H. Z.; Rai, R.; Cooper, Terrance.

In: Journal of bacteriology, Vol. 169, No. 4, 01.01.1987, p. 1684-1690.

Research output: Contribution to journalArticle

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