Regulation of apolipoprotein secretion by long-chain polyunsaturated fatty acids in newborn swine enterocytes

Heng Wang, Song Lu, Jianhui Du, Ying Yao, Helen M. Berschneider, Dennis Black

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Long-chain polyunsaturated fatty acids (LC-PUFA) are important in the development of the immature nervous system, and adding these fatty acids to infant formula has been proposed. To determine the effect of n-3 LC-PUFA on apolipoprotein secretion and lipid synthesis in newborn swine enterocytes, differentiated IPEC-1 cells were incubated for 24 h with docosahexaenoic acid (DHA; 22:6) or eicosapentaenoic acid (EPA; 20:5) complexed with albumin at a fatty acid concentration of 0.8 mM or albumin alone (control) added to the apical medium. Oleic acid (OA; 18:1) was used a control for lipid-labeling studies. Both DHA and EPA reduced apolipoprotien (apo) B secretion by one-half, whereas EPA increased apo A-I secretion. The increased apo A-I secretion occurred primarily in the high-density lipoprotein fraction. These changes in apoprotein secretion were not accompanied by significant changes in synthesis. Modest decreases in apo B mRNA levels were observed for DHA and EPA, whereas there were no changes in apo A-I mRNA abundance. EPA reduced cellular triacylglycerol labeling by one-half, and DHA and EPA decreased cellular phospholipid labeling compared with OA. Labeled triacylglycerol secretion was decreased 75% by EPA, and DHA doubled labeled phospholipid secretion. If present in vivo, these effects should be considered before supplementing infant formula with these fatty acids.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume280
Issue number6 43-6
StatePublished - Jul 3 2001

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Enterocytes
Apolipoproteins
Unsaturated Fatty Acids
Infant Formula
Swine
Fatty Acids
Albumins
Phospholipids
Triglycerides
Lipids
Messenger RNA
Apoproteins
Eicosapentaenoic Acid
Docosahexaenoic Acids
HDL Lipoproteins
Oleic Acid
Nervous System

All Science Journal Classification (ASJC) codes

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

Cite this

Regulation of apolipoprotein secretion by long-chain polyunsaturated fatty acids in newborn swine enterocytes. / Wang, Heng; Lu, Song; Du, Jianhui; Yao, Ying; Berschneider, Helen M.; Black, Dennis.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 280, No. 6 43-6, 03.07.2001.

Research output: Contribution to journalArticle

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