Regulation of CAD gene expression in mouse fibroblasts during the transition from the resting to the growing state

Rao Gadiparthi, Robert L. Church

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We have analyzed the steady-state levels of CAD mRNA and ATCase activity in BALB/c 3T3 mouse fibroblasts at quiescence and at various time points following the initiation of serum stimulation. Steady-state levels of CAD mRNA in 3T3 cells following 12 h of serum stimulation increased 10-fold over levels measured at quiescence. In contrast to the observed increase in steady-state levels of CAD mRNA, its rate of transcription increased only 3-fold, suggesting that the expression of CAD gene in these cells is regulated at both the transcriptional and post-transcriptional levels, to a major extent by the latter. These increases in CAD mRNA in serum-stimulated cells were followed by parallel increases in ATCase activity as well. When comparing DNA synthesis ([3H]thymidine uptake) to the accumulation of CAD mRNA and ATCase activity, it was observed that this accumulation occurred during the mid- to late-G1 phase of the cell cycle. These results suggest that the expression of CAD gene is cell growth dependent and may be a prerequisite to DNA synthesis.

Original languageEnglish (US)
Pages (from-to)449-456
Number of pages8
JournalExperimental Cell Research
Volume178
Issue number2
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

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Gene Expression Regulation
Fibroblasts
Messenger RNA
Serum
Gene Expression
3T3 Cells
DNA
G1 Phase
Thymidine
Cell Cycle
Growth

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Regulation of CAD gene expression in mouse fibroblasts during the transition from the resting to the growing state. / Gadiparthi, Rao; Church, Robert L.

In: Experimental Cell Research, Vol. 178, No. 2, 01.01.1988, p. 449-456.

Research output: Contribution to journalArticle

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