Regulation of delta opioid receptor expression by anti-CD3-ε, PMA, and ionomycin in murine splenocytes and T cells

Ming D. Li, Kathy McAllen, Burt Sharp

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Previous studies have shown that low levels of delta opioid receptor (DOR) mRNA were detectable by reverse transcription polymerase chain reaction (RT-PCR) in unstimulated splenocytes from BALB/c female mice. This study demonstrates that DOR transcripts can be detected in freshly obtained splenocytes from CD1 female mice as well. The results of studies using quantitative competitive RT-PCR showed that DOR transcripts in splenic T cells increased from < 1 copy/cell to 22 and 42 copies/cell, respectively, after stimulation with anti-CD3-ε for 24 and 48 h compared to the level in freshly obtained T cells. In the presence of actinomycin D, anti-CD3-ε did not affect the rate of degradation of DOR mRNA, suggesting that its stability is not altered by anti-CD3-ε. After incubation with phorbol myristate acetate (PMA) and ionomycin, the expression of DOR mRNA in splenocytes and T cells was significantly reduced compared with unstimulated cells in culture. In addition, the inhibitory effect of PMA prevented anti-CD3-ε-stimulated DOR expression. These data suggest that signaling through the T cell receptor complex by anti-CD3-ε regulates DOR expression differently than PMA and ionomycin.

Original languageEnglish (US)
Pages (from-to)707-714
Number of pages8
JournalJournal of Leukocyte Biology
Volume65
Issue number5
DOIs
StatePublished - Jan 1 1999

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delta Opioid Receptor
Ionomycin
Tetradecanoylphorbol Acetate
T-Lymphocytes
Messenger RNA
Reverse Transcription
CD3 Antigens
Polymerase Chain Reaction
Dactinomycin
T-Cell Antigen Receptor
Cell Culture Techniques

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Cell Biology

Cite this

Regulation of delta opioid receptor expression by anti-CD3-ε, PMA, and ionomycin in murine splenocytes and T cells. / Li, Ming D.; McAllen, Kathy; Sharp, Burt.

In: Journal of Leukocyte Biology, Vol. 65, No. 5, 01.01.1999, p. 707-714.

Research output: Contribution to journalArticle

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