Regulation of intestinal apolipoproteinA-I synthesis by dietary phosphatidylcholine in newborn swine

Heng Wang, Jianhui Du, Song Lu, Ying Yao, Felicia Hunter, Dennis Black

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Phospholipid (PL) from both dietary sources and biliary secretions may be important in the regulation of intestinal apolipoprotein (apo) synthesis. We previously demonstrated the up-regulation of apo A-I secretion by phosphatidylcholine (PC) in a newborn piglet intestinal epithelial cell line. We hypothesized that dietary PC increases small intestinal apo A-I synthesis in vivo in the newborn piglet. Two-day-old female swine were fed by gavage for 48 h. Diets consisted of a formula containing 51% of calories as triacylglycerol providing 180 kcal/kg/24 h. The experimental group (+PC, n = 7) received 1 g/L added soybean PC, and the control group (-PC, n = 7) received no added PC. At the end of the study period, jejunal apo A-I, B, and A-IV synthesis was measured, and apo A-I mRNA levels were quantitated. Jejunal mucosal PL content and serum lipids and apo B and A-I levels were measured. Jejunal apo A-I synthesis was almost twice as high in the +PC group as compared to the -PC group with no difference in apo A-I mRNA levels. Jejunal content of PL was higher in the +PC group than in the -PC group. There were no differences in jejunal apo B and A-IV synthesis or serum levels of lipids and apo-lipoproteins between the two groups. Dietary PC supplementation in newborn swine up-regulated jejunal apo A-I synthesis. Apo A-IV synthesis, which is sensitive to fatty acid flux, was not significantly increased, which suggests a specific effect of PC on apo A-I synthesis. Lumenal PC may be important in the regulation of intestinal apo A-I synthesis in the neonate.

Original languageEnglish (US)
Article number773
Pages (from-to)683-687
Number of pages5
JournalLipids
Volume36
Issue number7
DOIs
StatePublished - Jan 1 2001

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Phosphatidylcholines
Apolipoprotein A-I
Swine
Apolipoproteins A
Phospholipids
Apolipoproteins
Apolipoproteins B
Lipids
Messenger RNA
Nutrition
Dietary Supplements
Serum
Soybeans
Lipoproteins
Triglycerides
Up-Regulation
Fatty Acids
Epithelial Cells
Fluxes
Diet

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

Cite this

Regulation of intestinal apolipoproteinA-I synthesis by dietary phosphatidylcholine in newborn swine. / Wang, Heng; Du, Jianhui; Lu, Song; Yao, Ying; Hunter, Felicia; Black, Dennis.

In: Lipids, Vol. 36, No. 7, 773, 01.01.2001, p. 683-687.

Research output: Contribution to journalArticle

Wang, Heng ; Du, Jianhui ; Lu, Song ; Yao, Ying ; Hunter, Felicia ; Black, Dennis. / Regulation of intestinal apolipoproteinA-I synthesis by dietary phosphatidylcholine in newborn swine. In: Lipids. 2001 ; Vol. 36, No. 7. pp. 683-687.
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