Regulation of lipolysis and leptin biosynthesis in rodent adipose tissue by growth hormone

John N. Fain, Suleiman Bahouth

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The present study examined the effects of growth hormone (GH) on lipolysis and leptin release by cultured adipose tissue from rats and mice incubated for 24 hours in primary culture. A stimulation of leptin release by GH in rat adipose tissue was found in the presence of 25 nmol/L dexamethasone, and this was accompanied by a 28% increase in leptin mRNA content. GH stimulated lipolysis in rat adipose tissue in the presence of 0.1 nmol/L CL 316,243. In contrast, basal lipolysis in mouse adipose tissue was stimulated by GH, but this was not accompanied by an increase in leptin release. However, in the presence of insulin plus triiodothyronine (T3), the stimulation of lipolysis by GH was abolished and GH increased leptin release. These results indicate that GH can stimulate leptin release by both mouse and rat adipose tissue in the absence of a stimulation of lipolysis. In contrast, under conditions in which lipolysis is stimulated by GH, there is no effect on leptin release. (C) 2000 by W.B. Saunders Company.

Original languageEnglish (US)
Pages (from-to)239-244
Number of pages6
JournalMetabolism: Clinical and Experimental
Volume49
Issue number2
DOIs
StatePublished - Jan 1 2000

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Lipolysis
Leptin
Growth Hormone
Adipose Tissue
Rodentia
Triiodothyronine
Dexamethasone
Insulin
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Regulation of lipolysis and leptin biosynthesis in rodent adipose tissue by growth hormone. / Fain, John N.; Bahouth, Suleiman.

In: Metabolism: Clinical and Experimental, Vol. 49, No. 2, 01.01.2000, p. 239-244.

Research output: Contribution to journalArticle

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