Regulation of neuronal activation by alpha2A adrenergic receptor agonist

Valentina L. Savchenko, John Boughter

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Stress factors induce neuronal activation in brain areas that are related to anxiety and fear. High doses of caffeine induce neuronal activation with Ca 2+ influx followed by expression of the immediate early gene c-fos. In the present study, we investigated c-Fos protein expression in stress-responsive brain areas induced by caffeine, as well as the role of alpha2A receptor in the regulation of neuronal activation. Immunohistochemical analysis showed that an acute effect of caffeine induced c-Fos protein expression in the hippocampus, the bed nucleus of stria terminalis (BNST), the lateral septum, the basolateral and central amygdala, the paraventricular hypothalamic nucleus (PVN), the locus coeruleus, and the lateral parabrachial nucleus (LPBN). However, c-Fos expression was attenuated after repeated treatment of caffeine, spaced 24 h apart, compared to a single acute effect. Alpha2A receptor activation with the agonist guanfacine attenuated the acute effect of caffeine in terms of c-Fos expression in neurons in the CA1-CA3 areas of hippocampus, the locus coeruleus and the LPBN as compared with effect of caffeine alone, whereas the number of c-Fos expressing neurons increased in the lateral septum, the dorsal BNST, the central amygdala, and the PVN, areas that are densely innervated by noradrenergic neurons. Guanfacine alone induced c-Fos protein expression in neurons in the central amygdala, the dorsal BNST, the PVN, the LPBN, and the caudal nucleus of the solitary tract. Guanfacine alone also induced phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) in neurons expressing c-Fos in the dorsal BNST, the central amygdala, and the LPBN. These results suggest that alpha2A receptor activation modulates synaptic transmission in neuronal circuits that are correlated with stress in vivo.

Original languageEnglish (US)
Pages (from-to)226-239
Number of pages14
JournalNeurotoxicity Research
Volume20
Issue number3
DOIs
StatePublished - Oct 1 2011

Fingerprint

Adrenergic Agonists
Caffeine
Septal Nuclei
Neurons
Guanfacine
Chemical activation
Proto-Oncogene Proteins c-fos
Paraventricular Hypothalamic Nucleus
Locus Coeruleus
Hippocampus
Brain
Adrenergic Neurons
Phosphorylation
Solitary Nucleus
Immediate-Early Genes
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase 1
Synaptic Transmission
Fear
Anxiety

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Toxicology

Cite this

Regulation of neuronal activation by alpha2A adrenergic receptor agonist. / Savchenko, Valentina L.; Boughter, John.

In: Neurotoxicity Research, Vol. 20, No. 3, 01.10.2011, p. 226-239.

Research output: Contribution to journalArticle

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