Regulation of rapid eye movement sleep in the freely moving rat: Local microinjection of serotonin, norepinephrine, and adenosine into the brainstem

Subimal Datta, Vijayakumar Mavanji, Elissa H. Patterson, Jagadish Ulloor

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Study Objectives: Considerable evidence suggests that rapid eye movement (REM) sleep is induced by glutamatergic activation of cholinergic cells within the pedunculopontine tegmentum (PPT). The aim of this study is to test a popular hypothesis that serotonin, norepinephrine, and adenosine act on PPT cells to regulate REM sleep. This study also tests an alternate hypothesis that serotonin may inhibit REM sleep signs by direct action on the individual REM sleep sign generators. Design: Serotonin, norepinephrine, and adenosine were locally microinjected into the PPT and serotonin was microinjected into the pontine-wave (P-wave) generator (dorsal part of the locus subcoeruleus nucleus) while quantifying the effects on REM sleep and P-wave activity in freely moving rats. Setting: N/A. Participants: N/A. Interventions: N/A. Measurements and Results: Local microinjections of serotonin, norepinephrine, and adenosine into the PPT did not change REM sleep. Microinjection of serotonin into the P-wave generator suppressed P-wave activity but not REM sleep. Conclusions: The present findings provide direct evidence that serotonin, norepinephrine, and adenosine-induced REM sleep suppression in the behaving rat are not mediated by the PPT. The results also provide direct evidence, for the first time, that serotonin suppresses P-wave activity by acting directly on the P-wave generator. These results suggest that the serotonin-induced inhibition of REM sleep in the freely moving rat is probably not mediated through the mesopontine cholinergic cell compartment but, rather, through individual REM sleep sign generators. Citation: Datta S; Mavanji V; Patterson EH; Ulloor J. Regulation of rapid eye movement sleep in the freely moving rat: local microinjection of Serotonin, Norepinephrine, and Adenosine into the brainstem.

Original languageEnglish (US)
Pages (from-to)513-520
Number of pages8
JournalSleep
Volume26
Issue number5
DOIs
StatePublished - Aug 1 2003
Externally publishedYes

Fingerprint

REM Sleep
Microinjections
Adenosine
Brain Stem
Serotonin
Norepinephrine
Sleep
Cholinergic Agents

All Science Journal Classification (ASJC) codes

  • Clinical Neurology
  • Physiology (medical)

Cite this

Regulation of rapid eye movement sleep in the freely moving rat : Local microinjection of serotonin, norepinephrine, and adenosine into the brainstem. / Datta, Subimal; Mavanji, Vijayakumar; Patterson, Elissa H.; Ulloor, Jagadish.

In: Sleep, Vol. 26, No. 5, 01.08.2003, p. 513-520.

Research output: Contribution to journalArticle

Datta, Subimal ; Mavanji, Vijayakumar ; Patterson, Elissa H. ; Ulloor, Jagadish. / Regulation of rapid eye movement sleep in the freely moving rat : Local microinjection of serotonin, norepinephrine, and adenosine into the brainstem. In: Sleep. 2003 ; Vol. 26, No. 5. pp. 513-520.
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