Regulation of taurine transporter gene (TauT) by WT1

Xiaobin Han, Russell W. Chesney

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In the present study we have demonstrated that WT1 (Wilms tumor suppressor gene) enhances the expression of TauT (taurine transporter gene) in human embryonic kidney 293 cells in a dose-dependent manner. TauT promoter activity was increased five-fold by cotransfection of a full-length TauT promoter-reporter construct with WT1. Electrophoretic mobility shift assays (EMSAs) using nuclear extracts from WT1-overexpressing 293 cells showed a putative WT1-binding site in the basal promoter region of TauT, which bound to WT1 in EMSAs. Mutation of this WT1 consensus sequence abolished binding of WT1. These results demonstrate that TauT may represent a downstream target gene of WT1 during renal development.

Original languageEnglish (US)
Pages (from-to)71-76
Number of pages6
JournalFEBS Letters
Volume540
Issue number1-3
DOIs
StatePublished - Apr 10 2003

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Genes
Electrophoretic Mobility Shift Assay
Electrophoretic mobility
Wilms' Tumor Genes
Kidney
Assays
Consensus Sequence
Tumor Suppressor Genes
Reporter Genes
Genetic Promoter Regions
Binding Sites
taurine transporter
Gene Expression
Tumors
Mutation

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Regulation of taurine transporter gene (TauT) by WT1. / Han, Xiaobin; Chesney, Russell W.

In: FEBS Letters, Vol. 540, No. 1-3, 10.04.2003, p. 71-76.

Research output: Contribution to journalArticle

Han, Xiaobin ; Chesney, Russell W. / Regulation of taurine transporter gene (TauT) by WT1. In: FEBS Letters. 2003 ; Vol. 540, No. 1-3. pp. 71-76.
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