Regulation of the urea active transporter gene (DUR3) in Saccharomyces cerevisiae

H. M. ElBerry, M. L. Majumdar, T. S. Cunningham, R. A. Sumrada, Terrance Cooper

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The DUR3 gene, which encodes a component required for active transport of urea in Saccharomyces cerevisiae, has been isolated, and its sequence has been determined. The deduced DUR3 protein profile possesses alternating hydrophobic and hydrophilic regions characteristic of integral membrane proteins. Strong negative complementation observed during genetic analysis of the DUR3 locus suggests that the DUR3 product may polymerize to carry out its physiological function. Expression of DUR3 is regulated in a manner similar to that of other genes in the allantoin pathway. High-level expression is inducer dependent, requiring functional DAL81 and DAL82 genes. Maintenance of DUR3 mRNA at uninduced, nonrepressed basal levels requires the negatively acting DAL80 gene product. DUR3 expression is highly sensitive to nitrogen catabolite repression and also has a partial requirement for the GLN3 product.

Original languageEnglish (US)
Pages (from-to)4688-4698
Number of pages11
JournalJournal of bacteriology
Volume175
Issue number15
DOIs
StatePublished - Jan 1 1993

Fingerprint

Saccharomyces cerevisiae
Genes
Allantoin
Catabolite Repression
Active Biological Transport
Urea
Membrane Proteins
Nitrogen
Maintenance
Messenger RNA
urea transporter
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Regulation of the urea active transporter gene (DUR3) in Saccharomyces cerevisiae. / ElBerry, H. M.; Majumdar, M. L.; Cunningham, T. S.; Sumrada, R. A.; Cooper, Terrance.

In: Journal of bacteriology, Vol. 175, No. 15, 01.01.1993, p. 4688-4698.

Research output: Contribution to journalArticle

ElBerry, H. M. ; Majumdar, M. L. ; Cunningham, T. S. ; Sumrada, R. A. ; Cooper, Terrance. / Regulation of the urea active transporter gene (DUR3) in Saccharomyces cerevisiae. In: Journal of bacteriology. 1993 ; Vol. 175, No. 15. pp. 4688-4698.
@article{488150cde7124d18be460b8b706088ed,
title = "Regulation of the urea active transporter gene (DUR3) in Saccharomyces cerevisiae",
abstract = "The DUR3 gene, which encodes a component required for active transport of urea in Saccharomyces cerevisiae, has been isolated, and its sequence has been determined. The deduced DUR3 protein profile possesses alternating hydrophobic and hydrophilic regions characteristic of integral membrane proteins. Strong negative complementation observed during genetic analysis of the DUR3 locus suggests that the DUR3 product may polymerize to carry out its physiological function. Expression of DUR3 is regulated in a manner similar to that of other genes in the allantoin pathway. High-level expression is inducer dependent, requiring functional DAL81 and DAL82 genes. Maintenance of DUR3 mRNA at uninduced, nonrepressed basal levels requires the negatively acting DAL80 gene product. DUR3 expression is highly sensitive to nitrogen catabolite repression and also has a partial requirement for the GLN3 product.",
author = "ElBerry, {H. M.} and Majumdar, {M. L.} and Cunningham, {T. S.} and Sumrada, {R. A.} and Terrance Cooper",
year = "1993",
month = "1",
day = "1",
doi = "10.1128/jb.175.15.4688-4698.1993",
language = "English (US)",
volume = "175",
pages = "4688--4698",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "15",

}

TY - JOUR

T1 - Regulation of the urea active transporter gene (DUR3) in Saccharomyces cerevisiae

AU - ElBerry, H. M.

AU - Majumdar, M. L.

AU - Cunningham, T. S.

AU - Sumrada, R. A.

AU - Cooper, Terrance

PY - 1993/1/1

Y1 - 1993/1/1

N2 - The DUR3 gene, which encodes a component required for active transport of urea in Saccharomyces cerevisiae, has been isolated, and its sequence has been determined. The deduced DUR3 protein profile possesses alternating hydrophobic and hydrophilic regions characteristic of integral membrane proteins. Strong negative complementation observed during genetic analysis of the DUR3 locus suggests that the DUR3 product may polymerize to carry out its physiological function. Expression of DUR3 is regulated in a manner similar to that of other genes in the allantoin pathway. High-level expression is inducer dependent, requiring functional DAL81 and DAL82 genes. Maintenance of DUR3 mRNA at uninduced, nonrepressed basal levels requires the negatively acting DAL80 gene product. DUR3 expression is highly sensitive to nitrogen catabolite repression and also has a partial requirement for the GLN3 product.

AB - The DUR3 gene, which encodes a component required for active transport of urea in Saccharomyces cerevisiae, has been isolated, and its sequence has been determined. The deduced DUR3 protein profile possesses alternating hydrophobic and hydrophilic regions characteristic of integral membrane proteins. Strong negative complementation observed during genetic analysis of the DUR3 locus suggests that the DUR3 product may polymerize to carry out its physiological function. Expression of DUR3 is regulated in a manner similar to that of other genes in the allantoin pathway. High-level expression is inducer dependent, requiring functional DAL81 and DAL82 genes. Maintenance of DUR3 mRNA at uninduced, nonrepressed basal levels requires the negatively acting DAL80 gene product. DUR3 expression is highly sensitive to nitrogen catabolite repression and also has a partial requirement for the GLN3 product.

UR - http://www.scopus.com/inward/record.url?scp=0027172211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027172211&partnerID=8YFLogxK

U2 - 10.1128/jb.175.15.4688-4698.1993

DO - 10.1128/jb.175.15.4688-4698.1993

M3 - Article

VL - 175

SP - 4688

EP - 4698

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 15

ER -