Relative skeletal distribution of proliferating marrow in the adult dog determined using 3′-deoxy-3′-[18F]fluorothymidine

Joshua A. Rowe, Federica Morandi, Dustin Osborne, Jonathan Wall, Stephen Kennel, Robert B. Reed, Amy K. LeBlanc

Research output: Contribution to journalArticle

Abstract

3′-deoxy-3′-[18F]fluorothymidine (18FLT) is a radiopharmaceutical tracer used with positron emission tomography (PET), often in combination with computed tomography (CT), to image DNA synthesis, and thus, cellular proliferation. Characteristic accumulation of the tracer within haematopoietic bone marrow provides a noninvasive means to assess marrow activity and distribution throughout the living animal. The present study utilizes three-dimensional analysis of 18FLT-PET/CT scans to quantify the relative skeletal distribution of active marrow by anatomic site in the dog. Scans were performed on six healthy, adult (3–6 years of age), mixed-breed dogs using a commercially available PET/CT scanner consisting of a 64-slice helical CT scanner combined with an integrated four ring, high-resolution LSO PET scanner. Regions of interest encompassing 11 separate skeletal regions (skull, cervical vertebral column, thoracic vertebral column, lumbar vertebral column, sacrum, ribs, sternum, scapulae, proximal humeri, ossa coxarum, and proximal femora) were manually drawn based on CT images and thresholded by standardized uptake value to delineate bone marrow activity. Activity within each skeletal region was then divided by the total skeletal activity to derive the per cent of overall marrow activity within an individual site. The majority of proliferative marrow was located within the vertebral column. Of the sites traditionally accessed clinically for marrow sampling, the proximal humerus contained the largest percentage, followed by the ossa coxarum, proximal femur, and sternum, respectively. This information may be used to guide selection of traditional marrow sampling sites as well as inform efforts to spare important sites of haematopoiesis in radiation therapy planning.

Original languageEnglish (US)
Pages (from-to)46-52
Number of pages7
JournalJournal of Veterinary Medicine Series C: Anatomia Histologia Embryologia
Volume48
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

positron-emission tomography
computed tomography
spine (bones)
Bone Marrow
Dogs
scanners
dogs
sternum
humerus
Spine
femur
bone marrow
tracer techniques
Sternum
Humerus
scapula
hematopoiesis
Positron-Emission Tomography
Femur
radiotherapy

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

Relative skeletal distribution of proliferating marrow in the adult dog determined using 3′-deoxy-3′-[18F]fluorothymidine. / Rowe, Joshua A.; Morandi, Federica; Osborne, Dustin; Wall, Jonathan; Kennel, Stephen; Reed, Robert B.; LeBlanc, Amy K.

In: Journal of Veterinary Medicine Series C: Anatomia Histologia Embryologia, Vol. 48, No. 1, 01.01.2019, p. 46-52.

Research output: Contribution to journalArticle

@article{d78ed72743514a7a93bc8a2481c6df70,
title = "Relative skeletal distribution of proliferating marrow in the adult dog determined using 3′-deoxy-3′-[18F]fluorothymidine",
abstract = "3′-deoxy-3′-[18F]fluorothymidine (18FLT) is a radiopharmaceutical tracer used with positron emission tomography (PET), often in combination with computed tomography (CT), to image DNA synthesis, and thus, cellular proliferation. Characteristic accumulation of the tracer within haematopoietic bone marrow provides a noninvasive means to assess marrow activity and distribution throughout the living animal. The present study utilizes three-dimensional analysis of 18FLT-PET/CT scans to quantify the relative skeletal distribution of active marrow by anatomic site in the dog. Scans were performed on six healthy, adult (3–6 years of age), mixed-breed dogs using a commercially available PET/CT scanner consisting of a 64-slice helical CT scanner combined with an integrated four ring, high-resolution LSO PET scanner. Regions of interest encompassing 11 separate skeletal regions (skull, cervical vertebral column, thoracic vertebral column, lumbar vertebral column, sacrum, ribs, sternum, scapulae, proximal humeri, ossa coxarum, and proximal femora) were manually drawn based on CT images and thresholded by standardized uptake value to delineate bone marrow activity. Activity within each skeletal region was then divided by the total skeletal activity to derive the per cent of overall marrow activity within an individual site. The majority of proliferative marrow was located within the vertebral column. Of the sites traditionally accessed clinically for marrow sampling, the proximal humerus contained the largest percentage, followed by the ossa coxarum, proximal femur, and sternum, respectively. This information may be used to guide selection of traditional marrow sampling sites as well as inform efforts to spare important sites of haematopoiesis in radiation therapy planning.",
author = "Rowe, {Joshua A.} and Federica Morandi and Dustin Osborne and Jonathan Wall and Stephen Kennel and Reed, {Robert B.} and LeBlanc, {Amy K.}",
year = "2019",
month = "1",
day = "1",
doi = "10.1111/ahe.12410",
language = "English (US)",
volume = "48",
pages = "46--52",
journal = "Journal of Veterinary Medicine Series C: Anatomia Histologia Embryologia",
issn = "0340-2096",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Relative skeletal distribution of proliferating marrow in the adult dog determined using 3′-deoxy-3′-[18F]fluorothymidine

AU - Rowe, Joshua A.

AU - Morandi, Federica

AU - Osborne, Dustin

AU - Wall, Jonathan

AU - Kennel, Stephen

AU - Reed, Robert B.

AU - LeBlanc, Amy K.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - 3′-deoxy-3′-[18F]fluorothymidine (18FLT) is a radiopharmaceutical tracer used with positron emission tomography (PET), often in combination with computed tomography (CT), to image DNA synthesis, and thus, cellular proliferation. Characteristic accumulation of the tracer within haematopoietic bone marrow provides a noninvasive means to assess marrow activity and distribution throughout the living animal. The present study utilizes three-dimensional analysis of 18FLT-PET/CT scans to quantify the relative skeletal distribution of active marrow by anatomic site in the dog. Scans were performed on six healthy, adult (3–6 years of age), mixed-breed dogs using a commercially available PET/CT scanner consisting of a 64-slice helical CT scanner combined with an integrated four ring, high-resolution LSO PET scanner. Regions of interest encompassing 11 separate skeletal regions (skull, cervical vertebral column, thoracic vertebral column, lumbar vertebral column, sacrum, ribs, sternum, scapulae, proximal humeri, ossa coxarum, and proximal femora) were manually drawn based on CT images and thresholded by standardized uptake value to delineate bone marrow activity. Activity within each skeletal region was then divided by the total skeletal activity to derive the per cent of overall marrow activity within an individual site. The majority of proliferative marrow was located within the vertebral column. Of the sites traditionally accessed clinically for marrow sampling, the proximal humerus contained the largest percentage, followed by the ossa coxarum, proximal femur, and sternum, respectively. This information may be used to guide selection of traditional marrow sampling sites as well as inform efforts to spare important sites of haematopoiesis in radiation therapy planning.

AB - 3′-deoxy-3′-[18F]fluorothymidine (18FLT) is a radiopharmaceutical tracer used with positron emission tomography (PET), often in combination with computed tomography (CT), to image DNA synthesis, and thus, cellular proliferation. Characteristic accumulation of the tracer within haematopoietic bone marrow provides a noninvasive means to assess marrow activity and distribution throughout the living animal. The present study utilizes three-dimensional analysis of 18FLT-PET/CT scans to quantify the relative skeletal distribution of active marrow by anatomic site in the dog. Scans were performed on six healthy, adult (3–6 years of age), mixed-breed dogs using a commercially available PET/CT scanner consisting of a 64-slice helical CT scanner combined with an integrated four ring, high-resolution LSO PET scanner. Regions of interest encompassing 11 separate skeletal regions (skull, cervical vertebral column, thoracic vertebral column, lumbar vertebral column, sacrum, ribs, sternum, scapulae, proximal humeri, ossa coxarum, and proximal femora) were manually drawn based on CT images and thresholded by standardized uptake value to delineate bone marrow activity. Activity within each skeletal region was then divided by the total skeletal activity to derive the per cent of overall marrow activity within an individual site. The majority of proliferative marrow was located within the vertebral column. Of the sites traditionally accessed clinically for marrow sampling, the proximal humerus contained the largest percentage, followed by the ossa coxarum, proximal femur, and sternum, respectively. This information may be used to guide selection of traditional marrow sampling sites as well as inform efforts to spare important sites of haematopoiesis in radiation therapy planning.

UR - http://www.scopus.com/inward/record.url?scp=85055510163&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055510163&partnerID=8YFLogxK

U2 - 10.1111/ahe.12410

DO - 10.1111/ahe.12410

M3 - Article

VL - 48

SP - 46

EP - 52

JO - Journal of Veterinary Medicine Series C: Anatomia Histologia Embryologia

JF - Journal of Veterinary Medicine Series C: Anatomia Histologia Embryologia

SN - 0340-2096

IS - 1

ER -