Reproducibility of fasting plasma leptin concentration in lean and obese humans

Jianmei Liu, Hasan Askari, Samuel Dagogo-Jack

Research output: Contribution to journalArticle

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Abstract

We determined the reproducibility of plasma leptin levels in 20 healthy subjects (10 men, 10 women; 10 lean, 10 obese) at stable body weight. Blood samples were obtained, after an overnight fast, between 0700 and 0800 on days 1, 2, 3, 4, 5, 12, 19, and 26. Body weights were recorded on the same days. Plasma leptin was measured using a specific radioimmunoassay. The mean ± SE baseline body weights (kg) were 65.8 ± 3.6 (lean) and 96.4 ± 7.1 (obese). The body mass indices (BMI) were 22.9 ± 2.8 kg/m2 (lean) and 32.7 ± 2.2kg/m2 (obese). The mean daily fasting plasma glucose level was 98.7 ± 3.7 mg/dl. Baseline plasma leptin levels (ng/ml) were 5.3 ± 0.75 in lean men, 14.9 ± 4.6 in obese men, 11.2 ± 2.8 in lean women, and 27.1 ± 8.4 in obese women. Fasting leptin levels on days 2 to 26 were highly correlated with the baseline levels on day 1 (r2 = 0.9, P<0.0001). Body weights remained within 98% - 102% of baseline, whereas intra-individual leptin levels fluctuated between 80% and 120% of baseline values, throughout the 26 days of study. We conclude that fasting plasma leptin levels are reproducible, with a maximin day-to-day variation of ~20%, in healthy, free- living, lean and obese persons who maintain a stable body weight.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalEndocrine Research
Volume25
Issue number1
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

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Leptin
Fasting
Body Weight
Radioimmunoassay
Healthy Volunteers
Body Mass Index
Glucose

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Reproducibility of fasting plasma leptin concentration in lean and obese humans. / Liu, Jianmei; Askari, Hasan; Dagogo-Jack, Samuel.

In: Endocrine Research, Vol. 25, No. 1, 01.01.1999, p. 1-10.

Research output: Contribution to journalArticle

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