Resistance training increases heat shock protein levels in skeletal muscle of young and old rats

Zsolt Murlasits, Robert G. Cutlip, Kenneth B. Geronilla, K. Murali K. Rao, William F. Wonderlin, Stephen Alway

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Heat shock proteins (HSP) HSP72, HSC70 and HSP25 protein levels and mRNA levels of HSP72 genes (Hsp72-1, Hsp72-2, Hsp72-3) and HSC70 were examined in tibialis anterior muscles from young and old rats following 4.5 weeks of heavy resistance exercise. Young (3 months) (n=10) and old (30 months) (n=9) rats were subjected to 14 sessions of electrically evoked resistance training using stretch-shortening contractions of the left limb that activated the dorsiflexor muscle group, including the tibialis anterior muscle, while the right side served as the intra-animal control. Muscle wet weight of the left tibialis anterior increased by 15.6% in young animals compared to the untrained right side, while the aged rats demonstrated no significant hypertrophy based on muscle wet weight. There were no differences in mRNA expression between the control and experimental muscles in either the old or the young animals for any of the four genes examined. On the other hand, HSP72 levels as determined by Western blots were significantly (p<0.01) higher (968.8 and 409.1%) in the trained as compared to the contralateral control muscle in young and old animals, respectively. HSP25 expression was increased significantly (p<0.01) by training in muscles of young rats (943.1%) and old rats (420.3%). Moreover, there was no training by age interaction for HSP72, while a significant age and training by age effects were found in muscles for HSP25. There was no change in HSC70 protein expression in response to the training intervention in either age group. SOD-1 enzyme level increased by 66.6% in the trained muscles of the young rats, while this enzyme was 33% lower in trained muscles compared to the untrained control side in old rats. Moreover, a significant (p<0.05) training by age interaction was found for SOD-1 enzyme levels. This study suggests that fast contracting muscles in young and old animals are capable of increasing HSP expression in response to high intensity contractile stress. Furthermore, the data are consistent with the hypothesis that higher levels of oxidative stress in muscles of old animals limit HSP levels and/or function in response to high intensity contractile stress.

Original languageEnglish (US)
Pages (from-to)398-406
Number of pages9
JournalExperimental Gerontology
Volume41
Issue number4
DOIs
StatePublished - Apr 1 2006
Externally publishedYes

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Resistance Training
Heat-Shock Proteins
Muscle
Rats
Skeletal Muscle
Muscles
Animals
Enzymes
HSC70 Heat-Shock Proteins
Genes
HSP72 Heat-Shock Proteins
Weights and Measures
Messenger RNA
Oxidative stress
Hypertrophy
Proteins
Oxidative Stress
Extremities
Age Groups

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Aging
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Cell Biology

Cite this

Resistance training increases heat shock protein levels in skeletal muscle of young and old rats. / Murlasits, Zsolt; Cutlip, Robert G.; Geronilla, Kenneth B.; Rao, K. Murali K.; Wonderlin, William F.; Alway, Stephen.

In: Experimental Gerontology, Vol. 41, No. 4, 01.04.2006, p. 398-406.

Research output: Contribution to journalArticle

Murlasits, Zsolt ; Cutlip, Robert G. ; Geronilla, Kenneth B. ; Rao, K. Murali K. ; Wonderlin, William F. ; Alway, Stephen. / Resistance training increases heat shock protein levels in skeletal muscle of young and old rats. In: Experimental Gerontology. 2006 ; Vol. 41, No. 4. pp. 398-406.
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abstract = "Heat shock proteins (HSP) HSP72, HSC70 and HSP25 protein levels and mRNA levels of HSP72 genes (Hsp72-1, Hsp72-2, Hsp72-3) and HSC70 were examined in tibialis anterior muscles from young and old rats following 4.5 weeks of heavy resistance exercise. Young (3 months) (n=10) and old (30 months) (n=9) rats were subjected to 14 sessions of electrically evoked resistance training using stretch-shortening contractions of the left limb that activated the dorsiflexor muscle group, including the tibialis anterior muscle, while the right side served as the intra-animal control. Muscle wet weight of the left tibialis anterior increased by 15.6{\%} in young animals compared to the untrained right side, while the aged rats demonstrated no significant hypertrophy based on muscle wet weight. There were no differences in mRNA expression between the control and experimental muscles in either the old or the young animals for any of the four genes examined. On the other hand, HSP72 levels as determined by Western blots were significantly (p<0.01) higher (968.8 and 409.1{\%}) in the trained as compared to the contralateral control muscle in young and old animals, respectively. HSP25 expression was increased significantly (p<0.01) by training in muscles of young rats (943.1{\%}) and old rats (420.3{\%}). Moreover, there was no training by age interaction for HSP72, while a significant age and training by age effects were found in muscles for HSP25. There was no change in HSC70 protein expression in response to the training intervention in either age group. SOD-1 enzyme level increased by 66.6{\%} in the trained muscles of the young rats, while this enzyme was 33{\%} lower in trained muscles compared to the untrained control side in old rats. Moreover, a significant (p<0.05) training by age interaction was found for SOD-1 enzyme levels. This study suggests that fast contracting muscles in young and old animals are capable of increasing HSP expression in response to high intensity contractile stress. Furthermore, the data are consistent with the hypothesis that higher levels of oxidative stress in muscles of old animals limit HSP levels and/or function in response to high intensity contractile stress.",
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