Resistin release by human adipose tissue explants in primary culture

John N. Fain, Paramjeet S. Cheema, Suleiman Bahouth, M. Lloyd Hiler

Research output: Contribution to journalArticle

200 Citations (Scopus)

Abstract

Resistin, also known as Fizz3 or ADSF, is a protein found in murine adipose tissue and inflammatory lung exudates. The present studies found that resistin was released by explants of human adipose tissue but the release was quite variable ranging from 3 to 158ng/g over 48h. The release of resistin was 250% greater by explants of omental than by explants of human subcutaneous abdominal adipose tissue. Resistin release by adipocytes was negligible as compared to that by the non-fat cells of adipose tissue. Leptin formation by adipocytes was 8-fold greater than its formation by the non-fat cells, while the formation of PAI-1 by adipocytes was 38% of that by the non-fat cells. The conversion of glucose to lactate as well as the formation of PGE2 and IL-8 was approximately 15% of that by the non-fat cells. In contrast the release of IL-6 and IL-1β by adipocytes was 4-7% of that by the non-fat cells while the formation of resistin and IL-10 by adipocytes was 2% of that by non-fat cells. The release of adiponectin by explants ranged from 1000 to 5000ng/g over 48h but did not correlate with that of resistin. The present data suggest that resistin release by explants of human adipose tissue in primary culture is largely derived from the non-fat cells present in the explants.

Original languageEnglish (US)
Pages (from-to)674-678
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume300
Issue number3
DOIs
StatePublished - Jan 17 2003

Fingerprint

Resistin
Adipose Tissue
Tissue
Adipocytes
Abdominal Subcutaneous Fat
Adiponectin
Plasminogen Activator Inhibitor 1
Exudates and Transudates
Leptin
human RETN protein
Interleukin-8
Interleukin-1
Dinoprostone
Interleukin-10
Lactic Acid
Interleukin-6
Glucose
Lung

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Resistin release by human adipose tissue explants in primary culture. / Fain, John N.; Cheema, Paramjeet S.; Bahouth, Suleiman; Hiler, M. Lloyd.

In: Biochemical and Biophysical Research Communications, Vol. 300, No. 3, 17.01.2003, p. 674-678.

Research output: Contribution to journalArticle

Fain, John N. ; Cheema, Paramjeet S. ; Bahouth, Suleiman ; Hiler, M. Lloyd. / Resistin release by human adipose tissue explants in primary culture. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 300, No. 3. pp. 674-678.
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