Responses in extracellular and intracellular calcium and magnesium in aldosteronism

Aliye L. Runyan, Yao Sun, Syamal Bhattacharya, Robert A. Ahokas, Vikram S. Chhokar, Ivan Gerling, Karl Weber

Research output: Contribution to journalArticle

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Abstract

We hypothesized the hypercalciuria and hypermagnesuria that accompany aldosteronism could be pharmacologically attenuated to prevent shifts in extracellular and intracellular levels of these divalent cations and the adverse outcomes associated with them. Accordingly, rats administered aldosterone/salt treatment (ALDOST) were cotreated with either hydrochlorothiazide (Hctz), to selectively reabsorb urinary Ca2+, or with Hctz plus spironolactone (Hctz+Spi), where Spi retards the excretion of these cations in both urine and feces. We monitored urinary excretion and responses in extracellular and intracellular Ca2+ and Mg2+, together with indices of oxi/nitrosative stress in plasma and ventricular tissue. At 4 weeks ALDOST we found the following: (1) hypercalciuria was reduced by Hctz and normalized by Hctz+Spi, and this combination, unlike Hctz alone, also rescued hypermagnesuria; (2) the decrease in plasma-ionized [Ca2+]o was not seen with Hctz or Hctz+Spi, whereas Spi cotreatment protected against a decline in [Mg2+]o; (3) the Ca2+ loading of peripheral blood mononuclear cells and cardiac tissue was not seen with Hctz+Spi; and (4) the induction of oxi/nitrosative stress, expressed as reduced plasma α1-antiproteinase activity and activation of gp91 phox subunit of NADPH oxidase in inflammatory cells invading intramural coronary arteries of the right and left ventricles, together with vascular fibrosis, was completely prevented by Spi cotreatment. In rats with aldosteronism, cotreatment with Hctz+Spi more effectively (vis-à-vis Hctz alone) protects against adverse iterations in extracellular and intracellular concentrations of Ca2+ and Mg2+, as well as the appearance of oxi/nitrosative stress to prevent the proinflammatory vascular phenotype.

Original languageEnglish (US)
Pages (from-to)76-84
Number of pages9
JournalJournal of Laboratory and Clinical Medicine
Volume146
Issue number2
DOIs
StatePublished - Aug 1 2005

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Hyperaldosteronism
Hydrochlorothiazide
Magnesium
Calcium
Hypercalciuria
Aldosterone
Plasmas
Heart Ventricles
Blood Vessels
Rats
Salts
Tissue
NADPH Oxidase
Divalent Cations
Feces
Cations
Blood Cells
Coronary Vessels
Fibrosis
Blood

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Responses in extracellular and intracellular calcium and magnesium in aldosteronism. / Runyan, Aliye L.; Sun, Yao; Bhattacharya, Syamal; Ahokas, Robert A.; Chhokar, Vikram S.; Gerling, Ivan; Weber, Karl.

In: Journal of Laboratory and Clinical Medicine, Vol. 146, No. 2, 01.08.2005, p. 76-84.

Research output: Contribution to journalArticle

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