Responses of small intrapulmonary arteries to vasoactive compounds in the fetal and neonatal lamb

Norepinephrine, epinephrine, serotonin, and potassium chloride

Julie A. Dunn, Vichien Lorch, Sachchida N. Sinha

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Despite considerable study, the mechanisms responsible for the transition of the pulmonary circulation from the fetal to newborn life remain obscure. We compared the responses of third and fourth generation pulmonary arteries to norepinephrine, epinephrine, serotonin, and KC1 from lambs 7 d preterm and 1, 7, and 21 d of age to assess differences between age and third and fourth generations of the pulmonary arterial tree during the transitional period. Preterm vessels were significantly smaller in internal diameter than all other aged vessels for both generations. Fourth generation vessel response to KC1 increased with age (94 ± 15 mg/mm2, preterm; 259 ± 31 mg/mm2, 21 d). Third generation vessel response to KC1 did not change with age (135 ± 15 mg/mm2, preterm; 158 ±18 mg/mm2, 21 d). There were no differences in maximum response to norepinephrine and epinephrine between ages; however, third generation vessel response to these compounds was significantly greater (30-60% of maximum KC1 response) than fourth generation vessel response (010%). Third and fourth generation vessels had the same maximum response to serotonin regardless of age or generation. Third generation vessels were significantly reduced in sensitivity (log molar concentration which produced 50% of the maximum response—ECso) to norepinephrine at d 1 (5.89 ± 0.12 log molar) and 7 (5.90 + 0.21) compared to preterm (6.48 ± 0.10) and 21 d of age (6.50 ±.03). Both third and fourth generation vessels were less sensitive to serotonin at d 1 (5.04 ± 0.17, third; 5.61 ± 0.10, fourth) compared to d 7 (5.77 ± 0.13, third; 6.36 ± 0.12, fourth) and 21 d (5.82 ± 0.24, third; 6.50 ± 0.15, fourth). A similar nonsignificant trend occurred in third generation vessels to epinephrine at d 1 and 7 compared to vessels at preterm and 21 d of age. Greater than 75% of fourth generation vessels did not respond to norepinephrine and epinephrine. The small intrapulmonary arteries of the neonate are undergoing dynamic changes in physiologic response which are dependent upon age and generation.

Original languageEnglish (US)
Pages (from-to)360-363
Number of pages4
JournalPediatric Research
Volume25
Issue number4
DOIs
StatePublished - Jan 1 1989

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Potassium Chloride
Epinephrine
Serotonin
Norepinephrine
Arteries
Pulmonary Circulation
Pulmonary Artery
Lung

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health

Cite this

Responses of small intrapulmonary arteries to vasoactive compounds in the fetal and neonatal lamb : Norepinephrine, epinephrine, serotonin, and potassium chloride. / Dunn, Julie A.; Lorch, Vichien; Sinha, Sachchida N.

In: Pediatric Research, Vol. 25, No. 4, 01.01.1989, p. 360-363.

Research output: Contribution to journalArticle

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abstract = "Despite considerable study, the mechanisms responsible for the transition of the pulmonary circulation from the fetal to newborn life remain obscure. We compared the responses of third and fourth generation pulmonary arteries to norepinephrine, epinephrine, serotonin, and KC1 from lambs 7 d preterm and 1, 7, and 21 d of age to assess differences between age and third and fourth generations of the pulmonary arterial tree during the transitional period. Preterm vessels were significantly smaller in internal diameter than all other aged vessels for both generations. Fourth generation vessel response to KC1 increased with age (94 ± 15 mg/mm2, preterm; 259 ± 31 mg/mm2, 21 d). Third generation vessel response to KC1 did not change with age (135 ± 15 mg/mm2, preterm; 158 ±18 mg/mm2, 21 d). There were no differences in maximum response to norepinephrine and epinephrine between ages; however, third generation vessel response to these compounds was significantly greater (30-60{\%} of maximum KC1 response) than fourth generation vessel response (010{\%}). Third and fourth generation vessels had the same maximum response to serotonin regardless of age or generation. Third generation vessels were significantly reduced in sensitivity (log molar concentration which produced 50{\%} of the maximum response—ECso) to norepinephrine at d 1 (5.89 ± 0.12 log molar) and 7 (5.90 + 0.21) compared to preterm (6.48 ± 0.10) and 21 d of age (6.50 ±.03). Both third and fourth generation vessels were less sensitive to serotonin at d 1 (5.04 ± 0.17, third; 5.61 ± 0.10, fourth) compared to d 7 (5.77 ± 0.13, third; 6.36 ± 0.12, fourth) and 21 d (5.82 ± 0.24, third; 6.50 ± 0.15, fourth). A similar nonsignificant trend occurred in third generation vessels to epinephrine at d 1 and 7 compared to vessels at preterm and 21 d of age. Greater than 75{\%} of fourth generation vessels did not respond to norepinephrine and epinephrine. The small intrapulmonary arteries of the neonate are undergoing dynamic changes in physiologic response which are dependent upon age and generation.",
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N2 - Despite considerable study, the mechanisms responsible for the transition of the pulmonary circulation from the fetal to newborn life remain obscure. We compared the responses of third and fourth generation pulmonary arteries to norepinephrine, epinephrine, serotonin, and KC1 from lambs 7 d preterm and 1, 7, and 21 d of age to assess differences between age and third and fourth generations of the pulmonary arterial tree during the transitional period. Preterm vessels were significantly smaller in internal diameter than all other aged vessels for both generations. Fourth generation vessel response to KC1 increased with age (94 ± 15 mg/mm2, preterm; 259 ± 31 mg/mm2, 21 d). Third generation vessel response to KC1 did not change with age (135 ± 15 mg/mm2, preterm; 158 ±18 mg/mm2, 21 d). There were no differences in maximum response to norepinephrine and epinephrine between ages; however, third generation vessel response to these compounds was significantly greater (30-60% of maximum KC1 response) than fourth generation vessel response (010%). Third and fourth generation vessels had the same maximum response to serotonin regardless of age or generation. Third generation vessels were significantly reduced in sensitivity (log molar concentration which produced 50% of the maximum response—ECso) to norepinephrine at d 1 (5.89 ± 0.12 log molar) and 7 (5.90 + 0.21) compared to preterm (6.48 ± 0.10) and 21 d of age (6.50 ±.03). Both third and fourth generation vessels were less sensitive to serotonin at d 1 (5.04 ± 0.17, third; 5.61 ± 0.10, fourth) compared to d 7 (5.77 ± 0.13, third; 6.36 ± 0.12, fourth) and 21 d (5.82 ± 0.24, third; 6.50 ± 0.15, fourth). A similar nonsignificant trend occurred in third generation vessels to epinephrine at d 1 and 7 compared to vessels at preterm and 21 d of age. Greater than 75% of fourth generation vessels did not respond to norepinephrine and epinephrine. The small intrapulmonary arteries of the neonate are undergoing dynamic changes in physiologic response which are dependent upon age and generation.

AB - Despite considerable study, the mechanisms responsible for the transition of the pulmonary circulation from the fetal to newborn life remain obscure. We compared the responses of third and fourth generation pulmonary arteries to norepinephrine, epinephrine, serotonin, and KC1 from lambs 7 d preterm and 1, 7, and 21 d of age to assess differences between age and third and fourth generations of the pulmonary arterial tree during the transitional period. Preterm vessels were significantly smaller in internal diameter than all other aged vessels for both generations. Fourth generation vessel response to KC1 increased with age (94 ± 15 mg/mm2, preterm; 259 ± 31 mg/mm2, 21 d). Third generation vessel response to KC1 did not change with age (135 ± 15 mg/mm2, preterm; 158 ±18 mg/mm2, 21 d). There were no differences in maximum response to norepinephrine and epinephrine between ages; however, third generation vessel response to these compounds was significantly greater (30-60% of maximum KC1 response) than fourth generation vessel response (010%). Third and fourth generation vessels had the same maximum response to serotonin regardless of age or generation. Third generation vessels were significantly reduced in sensitivity (log molar concentration which produced 50% of the maximum response—ECso) to norepinephrine at d 1 (5.89 ± 0.12 log molar) and 7 (5.90 + 0.21) compared to preterm (6.48 ± 0.10) and 21 d of age (6.50 ±.03). Both third and fourth generation vessels were less sensitive to serotonin at d 1 (5.04 ± 0.17, third; 5.61 ± 0.10, fourth) compared to d 7 (5.77 ± 0.13, third; 6.36 ± 0.12, fourth) and 21 d (5.82 ± 0.24, third; 6.50 ± 0.15, fourth). A similar nonsignificant trend occurred in third generation vessels to epinephrine at d 1 and 7 compared to vessels at preterm and 21 d of age. Greater than 75% of fourth generation vessels did not respond to norepinephrine and epinephrine. The small intrapulmonary arteries of the neonate are undergoing dynamic changes in physiologic response which are dependent upon age and generation.

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