Restricted TCR Vα Repertoire in the T Cell Response to a Tolerogenic Determinant of Type II Collagen

Seiichi Kawamura, Hisaaki Miyahara, Yukio Esaki, Linda Myers, Edward F. Rosloniec, John Stuart, Andrew Kang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Tolerization of B10.RIII mice (H-2r) with i.v.-injected type II collagen (CII) renders the animals resistant to induction of collagen-induced arthritis (CIA), The B10.RIII mouse is of particular interest, in that the T cell determinants that induce tolerance are different from those that induce arthritis. To characterize T cells that react with the tolerogenic determinant and play a role in regulation of arthritis, we have developed a panel of T cell hybridomas reactive with the tolerogenic T cell epitope, CII 607-621. None of the hybrids cross-reacted with either the arthritogenic CII 445-453 or murine CII. As determined by PCR and immunofluorescence, the T cell response to the tolerogenic determinant was oligoclonal, with evident preferential usage of Vα. Through the analysis of a large panel of T cell hybridomas, preferential usage of Vα2, Jα44, Jβ2.7, and Dβ2.1 was observed. Characterization of T cells reactive with the immunodominant determinant, CII 607-621, responsible for the induction of tolerance should prove important in developing novel therapeutic approaches for the treatment of autoimmune diseases.

Original languageEnglish (US)
Pages (from-to)6013-6018
Number of pages6
JournalJournal of Immunology
Volume158
Issue number12
StatePublished - Jun 15 1997

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Collagen Type II
T-Lymphocytes
Hybridomas
Arthritis
Immunodominant Epitopes
T-Lymphocyte Epitopes
Experimental Arthritis
Autoimmune Diseases
Fluorescent Antibody Technique
Polymerase Chain Reaction
Therapeutics

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Kawamura, S., Miyahara, H., Esaki, Y., Myers, L., Rosloniec, E. F., Stuart, J., & Kang, A. (1997). Restricted TCR Vα Repertoire in the T Cell Response to a Tolerogenic Determinant of Type II Collagen. Journal of Immunology, 158(12), 6013-6018.

Restricted TCR Vα Repertoire in the T Cell Response to a Tolerogenic Determinant of Type II Collagen. / Kawamura, Seiichi; Miyahara, Hisaaki; Esaki, Yukio; Myers, Linda; Rosloniec, Edward F.; Stuart, John; Kang, Andrew.

In: Journal of Immunology, Vol. 158, No. 12, 15.06.1997, p. 6013-6018.

Research output: Contribution to journalArticle

Kawamura, S, Miyahara, H, Esaki, Y, Myers, L, Rosloniec, EF, Stuart, J & Kang, A 1997, 'Restricted TCR Vα Repertoire in the T Cell Response to a Tolerogenic Determinant of Type II Collagen', Journal of Immunology, vol. 158, no. 12, pp. 6013-6018.
Kawamura S, Miyahara H, Esaki Y, Myers L, Rosloniec EF, Stuart J et al. Restricted TCR Vα Repertoire in the T Cell Response to a Tolerogenic Determinant of Type II Collagen. Journal of Immunology. 1997 Jun 15;158(12):6013-6018.
Kawamura, Seiichi ; Miyahara, Hisaaki ; Esaki, Yukio ; Myers, Linda ; Rosloniec, Edward F. ; Stuart, John ; Kang, Andrew. / Restricted TCR Vα Repertoire in the T Cell Response to a Tolerogenic Determinant of Type II Collagen. In: Journal of Immunology. 1997 ; Vol. 158, No. 12. pp. 6013-6018.
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