Resveratrol inhibits nitric oxide-induced apoptosis via the nf-kappa B pathway in rabbit articular chondrocytes

Seong Hui Eo, Hongsik Cho, Song Ja Kim

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Resveratrol (trans-3,4'-trihydroxystillbene), a naturally occurring polyphenolic antioxidant found in grapes and red wine, elicits diverse biochemical responses and demonstrates anti-aging, anti-inflammatory, and anti-proliferative effects in several cell types. Previously, resveratrol was shown to regulate differentiation and inflammation in rabbit articular chondrocytes, while the direct production of nitric oxide (NO) in these cells by treatment with the NO donor sodium nitroprusside (SNP) led to apoptosis. In this study, the effect of resveratrol on NO-induced apoptosis in rabbit articular chondrocytes was investigated. Resveratrol dramatically reduced NO-induced apoptosis in chondrocytes, as determined by phase-contrast microscopy, the MTT assay, FACS analysis, and DAPI staining. Treatment with resveratrol inhibited the SNP-induced expression of p53 and p21 and reduced the expression of procaspase-3 in chondrocytes, as detected by western blot analysis. SNP-induced degradation of I-kappa B alpha (IκB-α) was rescued by resveratrol treatment, and the SN50 peptide-mediated inhibition of NF-kappa B (NF-κB) activity potently blocked SNP-induced caspase-3 activation and apoptosis. Our results suggest that resveratrol inhibits NO-induced apoptosis through the NF-κB pathway in articular chondrocytes.

Original languageEnglish (US)
Pages (from-to)364-370
Number of pages7
JournalBiomolecules and Therapeutics
Volume21
Issue number5
DOIs
StatePublished - Nov 15 2013

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Chondrocytes
Nitric Oxide
Joints
Apoptosis
Rabbits
Nitroprusside
NF-kappa B
Caspase 3
I-kappa B Proteins
Phase-Contrast Microscopy
Wine
Nitric Oxide Donors
Vitis
resveratrol
Assays
Microscopic examination
Anti-Inflammatory Agents
Antioxidants
Aging of materials
Western Blotting

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Pharmacology
  • Drug Discovery

Cite this

Resveratrol inhibits nitric oxide-induced apoptosis via the nf-kappa B pathway in rabbit articular chondrocytes. / Eo, Seong Hui; Cho, Hongsik; Kim, Song Ja.

In: Biomolecules and Therapeutics, Vol. 21, No. 5, 15.11.2013, p. 364-370.

Research output: Contribution to journalArticle

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