Retinol dehydrogenases RDH11 and RDH12 in the mouse retina

Expression levels during development and regulation by oxidative stress

Yogita Kanan, Lea D. Wicker, Muayyad R. Al-Ubaidi, Nawajes Mandal, Anne Kasus-Jacobi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

PURPOSE. RDH11 and RDH12 are closely related retinol dehydrogenases expressed in the retina. RDH12 has been linked to the early-onset retinal dystrophy Leber congenital amaurosis, whereas RDH11 has not been associated with human disease. To understand their physiological roles, the authors investigated their expression during development and their regulation by light-induced oxidative stress in mouse retina. METHODS. Quantitative RT-PCR and immunoblot analysis were used for quantification of RDH11 and RDH12 during development and oxidative stress. Expression during development was measured between embryonic day (E) 12 and postnatal day (P) 210 (7 months) in C57BL/6 mouse eyes. Expression during light-induced oxidative stress was measured between 2 and 24 hours of exposure to light in BALB/c mouse retina. RESULTS. The RDH11 level was low and remarkably constant during development and oxidative stress. RDH12 expression started at P7 and increased until P30 to approximately sevenfold higher than RDH11. Oxidative stress induced by exposure to constant bright light led to a rapid and significant decrease of RDH12 protein. CONCLUSIONS. The low and constant expression of RDH11 suggested a housekeeping function for this enzyme. The onset of RDH12 expression during the maturation of photoreceptor cells suggested a function related to the visual process. The light-induced rapid decrease of RDH12 protein, preceding the decrease of the mRNA, suggested a specific degradation of the orotein rather than a regulation of gene expression.

Original languageEnglish (US)
Pages (from-to)1071-1078
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume49
Issue number3
DOIs
StatePublished - Mar 1 2008
Externally publishedYes

Fingerprint

Retina
Oxidative Stress
Light
Leber Congenital Amaurosis
Retinal Dystrophies
Housekeeping
Photoreceptor Cells
Gene Expression Regulation
Inbred C57BL Mouse
Proteins
retinol dehydrogenase
Polymerase Chain Reaction
Messenger RNA
Enzymes

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Retinol dehydrogenases RDH11 and RDH12 in the mouse retina : Expression levels during development and regulation by oxidative stress. / Kanan, Yogita; Wicker, Lea D.; Al-Ubaidi, Muayyad R.; Mandal, Nawajes; Kasus-Jacobi, Anne.

In: Investigative Ophthalmology and Visual Science, Vol. 49, No. 3, 01.03.2008, p. 1071-1078.

Research output: Contribution to journalArticle

@article{afb53895793a4d1e9412c5bd31d17f4b,
title = "Retinol dehydrogenases RDH11 and RDH12 in the mouse retina: Expression levels during development and regulation by oxidative stress",
abstract = "PURPOSE. RDH11 and RDH12 are closely related retinol dehydrogenases expressed in the retina. RDH12 has been linked to the early-onset retinal dystrophy Leber congenital amaurosis, whereas RDH11 has not been associated with human disease. To understand their physiological roles, the authors investigated their expression during development and their regulation by light-induced oxidative stress in mouse retina. METHODS. Quantitative RT-PCR and immunoblot analysis were used for quantification of RDH11 and RDH12 during development and oxidative stress. Expression during development was measured between embryonic day (E) 12 and postnatal day (P) 210 (7 months) in C57BL/6 mouse eyes. Expression during light-induced oxidative stress was measured between 2 and 24 hours of exposure to light in BALB/c mouse retina. RESULTS. The RDH11 level was low and remarkably constant during development and oxidative stress. RDH12 expression started at P7 and increased until P30 to approximately sevenfold higher than RDH11. Oxidative stress induced by exposure to constant bright light led to a rapid and significant decrease of RDH12 protein. CONCLUSIONS. The low and constant expression of RDH11 suggested a housekeeping function for this enzyme. The onset of RDH12 expression during the maturation of photoreceptor cells suggested a function related to the visual process. The light-induced rapid decrease of RDH12 protein, preceding the decrease of the mRNA, suggested a specific degradation of the orotein rather than a regulation of gene expression.",
author = "Yogita Kanan and Wicker, {Lea D.} and Al-Ubaidi, {Muayyad R.} and Nawajes Mandal and Anne Kasus-Jacobi",
year = "2008",
month = "3",
day = "1",
doi = "10.1167/iovs.07-1207",
language = "English (US)",
volume = "49",
pages = "1071--1078",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "3",

}

TY - JOUR

T1 - Retinol dehydrogenases RDH11 and RDH12 in the mouse retina

T2 - Expression levels during development and regulation by oxidative stress

AU - Kanan, Yogita

AU - Wicker, Lea D.

AU - Al-Ubaidi, Muayyad R.

AU - Mandal, Nawajes

AU - Kasus-Jacobi, Anne

PY - 2008/3/1

Y1 - 2008/3/1

N2 - PURPOSE. RDH11 and RDH12 are closely related retinol dehydrogenases expressed in the retina. RDH12 has been linked to the early-onset retinal dystrophy Leber congenital amaurosis, whereas RDH11 has not been associated with human disease. To understand their physiological roles, the authors investigated their expression during development and their regulation by light-induced oxidative stress in mouse retina. METHODS. Quantitative RT-PCR and immunoblot analysis were used for quantification of RDH11 and RDH12 during development and oxidative stress. Expression during development was measured between embryonic day (E) 12 and postnatal day (P) 210 (7 months) in C57BL/6 mouse eyes. Expression during light-induced oxidative stress was measured between 2 and 24 hours of exposure to light in BALB/c mouse retina. RESULTS. The RDH11 level was low and remarkably constant during development and oxidative stress. RDH12 expression started at P7 and increased until P30 to approximately sevenfold higher than RDH11. Oxidative stress induced by exposure to constant bright light led to a rapid and significant decrease of RDH12 protein. CONCLUSIONS. The low and constant expression of RDH11 suggested a housekeeping function for this enzyme. The onset of RDH12 expression during the maturation of photoreceptor cells suggested a function related to the visual process. The light-induced rapid decrease of RDH12 protein, preceding the decrease of the mRNA, suggested a specific degradation of the orotein rather than a regulation of gene expression.

AB - PURPOSE. RDH11 and RDH12 are closely related retinol dehydrogenases expressed in the retina. RDH12 has been linked to the early-onset retinal dystrophy Leber congenital amaurosis, whereas RDH11 has not been associated with human disease. To understand their physiological roles, the authors investigated their expression during development and their regulation by light-induced oxidative stress in mouse retina. METHODS. Quantitative RT-PCR and immunoblot analysis were used for quantification of RDH11 and RDH12 during development and oxidative stress. Expression during development was measured between embryonic day (E) 12 and postnatal day (P) 210 (7 months) in C57BL/6 mouse eyes. Expression during light-induced oxidative stress was measured between 2 and 24 hours of exposure to light in BALB/c mouse retina. RESULTS. The RDH11 level was low and remarkably constant during development and oxidative stress. RDH12 expression started at P7 and increased until P30 to approximately sevenfold higher than RDH11. Oxidative stress induced by exposure to constant bright light led to a rapid and significant decrease of RDH12 protein. CONCLUSIONS. The low and constant expression of RDH11 suggested a housekeeping function for this enzyme. The onset of RDH12 expression during the maturation of photoreceptor cells suggested a function related to the visual process. The light-induced rapid decrease of RDH12 protein, preceding the decrease of the mRNA, suggested a specific degradation of the orotein rather than a regulation of gene expression.

UR - http://www.scopus.com/inward/record.url?scp=41949121917&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=41949121917&partnerID=8YFLogxK

U2 - 10.1167/iovs.07-1207

DO - 10.1167/iovs.07-1207

M3 - Article

VL - 49

SP - 1071

EP - 1078

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 3

ER -