Retroviral mediated gene transduction alters integrin expression on vascular endothelial cells

Jill E. Sackman, Ann M. Wymore, Tonya T. Reddick, Michael Freeman, Scott Stevens, Mitchell Goldman

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Genetically recombinant endothelial cells (rEC) may improve the patency of small diameter vascular grafts by preventing thrombosis or limiting neointimal hyperplasia. Previous work has shown that rEC have reduced adhesion to vascular bypass grafts in vivo. Poor adhesion may be due to altered adhesion (integrin) receptors. This study evaluated the expression of the α5β1 (fibronectin), α2β1 (collagen IV), and α(v)β3 (vitronectin) integrin subunits on rEC. Human umbilical vein EC or canine jugular vein EC were transduced with neoR, neoR and human tPA or hygromycin resistance genes using retrovital vectors. Naive EC and EC exposed to empty viral particles (mEC) were controls. Naive EC, mEC, and all rEC's were evaluated for α and β subunits for each integrin receptor studied using immunoblotting. Blotting for α2, α5 and α(v) exhibited expression of the α integrin subunits in all cells. The β and β3 subunits were present in mEC and nEC but were absent or truncated in all rEC. The decreased adhesion of rEC's to synthetic vascular grafts may be accounted for by their altered β1 and β3 integrin subunit expression. The β subunit is critical for organization of the cytoskeleton and cellular signal transduction. Diminished β subunit expression in rEC is neither vector specific nor related to retrovital exposure alone. Alteration of β integrin expression may be to associated with the over-expression of phosphotransferase genes such as neoR or hygromycin B used as selectable markers in gene transfer protocols.

Original languageEnglish (US)
Pages (from-to)45-50
Number of pages6
JournalJournal of Surgical Research
Volume69
Issue number1
DOIs
StatePublished - Jan 1 1997

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Integrins
Endothelial Cells
Genes
Blood Vessels
Transplants
Hygromycin B
Vitronectin
Umbilical Veins
Jugular Veins
Cytoskeleton
Fibronectins
Immunoblotting
Virion
Hyperplasia
Canidae
Signal Transduction
Thrombosis
Phosphotransferases
Collagen
Gene Expression

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Retroviral mediated gene transduction alters integrin expression on vascular endothelial cells. / Sackman, Jill E.; Wymore, Ann M.; Reddick, Tonya T.; Freeman, Michael; Stevens, Scott; Goldman, Mitchell.

In: Journal of Surgical Research, Vol. 69, No. 1, 01.01.1997, p. 45-50.

Research output: Contribution to journalArticle

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