Retrovirally mediated telomerase immortalization of neural progenitor cells

Neeta Roy, Devin Chandler-Militello, Gang Lu, Su Wang

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Traditional methods of generating immortalized lines of both somatic cells and their progenitors have relied on the use of oncogenes. However, the resulting lines are typically anaplastic in vitro and tumorigenic in vivo, and hence of limited utility. The overexpression of telomerase, as mediated by the induced overexpression of human telomerase reverse transcriptase (hTERT), has permitted the generation of stable, non-oncogenic lines of a variety of cell types. This strategy for immortalization has found special utility in the central nervous system, as few stable lines are available for the study of either human neural progenitor cells, or of neurons or glia of restricted phenotype. We describe the use of retroviral hTERT overexpression for generating lines of immortalized human neural progenitor cells, whose neuronal progeny are phenotypically restricted, post-mitotic and functionally competent. Although we focus here on telomerase immortalization of spinal neural progenitors, this is a broadly applicable protocol for using hTERT to immortalize human fetal neural progenitors of any pre-selected phenotype and for characterizing the cell lines thereby generated.

Original languageEnglish (US)
Pages (from-to)2815-2825
Number of pages11
JournalNature Protocols
Volume2
Issue number11
DOIs
StatePublished - Nov 15 2007

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Telomerase
Stem Cells
Phenotype
Neurology
Oncogenes
Neuroglia
Neurons
Central Nervous System
Cells
Cell Line
human TERT protein

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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Retrovirally mediated telomerase immortalization of neural progenitor cells. / Roy, Neeta; Chandler-Militello, Devin; Lu, Gang; Wang, Su.

In: Nature Protocols, Vol. 2, No. 11, 15.11.2007, p. 2815-2825.

Research output: Contribution to journalArticle

Roy, Neeta ; Chandler-Militello, Devin ; Lu, Gang ; Wang, Su. / Retrovirally mediated telomerase immortalization of neural progenitor cells. In: Nature Protocols. 2007 ; Vol. 2, No. 11. pp. 2815-2825.
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