RGS14 is a natural suppressor of both synaptic plasticity in CA2 neurons and hippocampal-based learning and memory

Sarah Emerson Lee, Stephen B. Simons, Scott Heldt, Meilan Zhao, Jason P. Schroeder, Christopher P. Vellano, D. Patrick Cowan, Suneela Ramineni, Cindee K. Yates, Yue Feng, Yoland Smith, J. David Sweatt, David Weinshenker, Kerry J. Ressler, Serena M. Dudek, John R. Hepler

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Abstract

Learning and memory have been closely linked to strengthening of synaptic connections between neurons (i.e., synaptic plasticity) within the dentate gyrus (DG)-CA3-CA1 trisynaptic circuit of the hippocampus. Conspicuously absent from this circuit is area CA2, an intervening hippocampal region that is poorly understood. Schaffer collateral synapses on CA2 neurons are distinct from those on other hippocampal neurons in that they exhibit a perplexing lack of synaptic long-term potentiation (LTP). Here we demonstrate that the signaling protein RGS14 is highly enriched in CA2 pyramidal neurons and plays a role in suppression of both synaptic plasticity at these synapses and hippocampal-based learning and memory. RGS14 is a scaffolding protein that integrates G protein and H-Ras/ ERK/MAP kinase signaling pathways, thereby making it well positioned to suppress plasticity in CA2 neurons. Supporting this idea, deletion of exons 2-7 of the RGS14 gene yields mice that lack RGS14 (RGS14-KO) and now express robust LTP at glutamatergic synapses in CA2 neurons with no impact on synaptic plasticity in CA1 neurons. Treatment of RGS14-deficient CA2 neurons with a specific MEK inhibitor blocked this LTP, suggesting a role for ERK/MAP kinase signaling pathways in this process. When tested behaviorally, RGS14-KO mice exhibited marked enhancement in spatial learning and in object recognition memory compared with their wild-type littermates, but showed no differences in their performance on tests of nonhippocampal-dependent behaviors. These results demonstrate that RGS14 is a key regulator of signaling pathways linking synaptic plasticity in CA2 pyramidal neurons to hippocampal-based learning and memory but distinct from the canonical DG-CA3-CA1 circuit.

Original languageEnglish (US)
Pages (from-to)16994-16998
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number39
DOIs
StatePublished - Sep 28 2010

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Neuronal Plasticity
Learning
Neurons
Long-Term Potentiation
Synapses
MAP Kinase Signaling System
Pyramidal Cells
Extracellular Signal-Regulated MAP Kinases
Dentate Gyrus
Hippocampal CA2 Region
Hippocampus
Mitogen-Activated Protein Kinase Kinases
GTP-Binding Proteins
Exons
Proteins
Genes

All Science Journal Classification (ASJC) codes

  • General

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RGS14 is a natural suppressor of both synaptic plasticity in CA2 neurons and hippocampal-based learning and memory. / Lee, Sarah Emerson; Simons, Stephen B.; Heldt, Scott; Zhao, Meilan; Schroeder, Jason P.; Vellano, Christopher P.; Cowan, D. Patrick; Ramineni, Suneela; Yates, Cindee K.; Feng, Yue; Smith, Yoland; David Sweatt, J.; Weinshenker, David; Ressler, Kerry J.; Dudek, Serena M.; Hepler, John R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 39, 28.09.2010, p. 16994-16998.

Research output: Contribution to journalArticle

Lee, SE, Simons, SB, Heldt, S, Zhao, M, Schroeder, JP, Vellano, CP, Cowan, DP, Ramineni, S, Yates, CK, Feng, Y, Smith, Y, David Sweatt, J, Weinshenker, D, Ressler, KJ, Dudek, SM & Hepler, JR 2010, 'RGS14 is a natural suppressor of both synaptic plasticity in CA2 neurons and hippocampal-based learning and memory', Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 39, pp. 16994-16998. https://doi.org/10.1073/pnas.1005362107
Lee, Sarah Emerson ; Simons, Stephen B. ; Heldt, Scott ; Zhao, Meilan ; Schroeder, Jason P. ; Vellano, Christopher P. ; Cowan, D. Patrick ; Ramineni, Suneela ; Yates, Cindee K. ; Feng, Yue ; Smith, Yoland ; David Sweatt, J. ; Weinshenker, David ; Ressler, Kerry J. ; Dudek, Serena M. ; Hepler, John R. / RGS14 is a natural suppressor of both synaptic plasticity in CA2 neurons and hippocampal-based learning and memory. In: Proceedings of the National Academy of Sciences of the United States of America. 2010 ; Vol. 107, No. 39. pp. 16994-16998.
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abstract = "Learning and memory have been closely linked to strengthening of synaptic connections between neurons (i.e., synaptic plasticity) within the dentate gyrus (DG)-CA3-CA1 trisynaptic circuit of the hippocampus. Conspicuously absent from this circuit is area CA2, an intervening hippocampal region that is poorly understood. Schaffer collateral synapses on CA2 neurons are distinct from those on other hippocampal neurons in that they exhibit a perplexing lack of synaptic long-term potentiation (LTP). Here we demonstrate that the signaling protein RGS14 is highly enriched in CA2 pyramidal neurons and plays a role in suppression of both synaptic plasticity at these synapses and hippocampal-based learning and memory. RGS14 is a scaffolding protein that integrates G protein and H-Ras/ ERK/MAP kinase signaling pathways, thereby making it well positioned to suppress plasticity in CA2 neurons. Supporting this idea, deletion of exons 2-7 of the RGS14 gene yields mice that lack RGS14 (RGS14-KO) and now express robust LTP at glutamatergic synapses in CA2 neurons with no impact on synaptic plasticity in CA1 neurons. Treatment of RGS14-deficient CA2 neurons with a specific MEK inhibitor blocked this LTP, suggesting a role for ERK/MAP kinase signaling pathways in this process. When tested behaviorally, RGS14-KO mice exhibited marked enhancement in spatial learning and in object recognition memory compared with their wild-type littermates, but showed no differences in their performance on tests of nonhippocampal-dependent behaviors. These results demonstrate that RGS14 is a key regulator of signaling pathways linking synaptic plasticity in CA2 pyramidal neurons to hippocampal-based learning and memory but distinct from the canonical DG-CA3-CA1 circuit.",
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T1 - RGS14 is a natural suppressor of both synaptic plasticity in CA2 neurons and hippocampal-based learning and memory

AU - Lee, Sarah Emerson

AU - Simons, Stephen B.

AU - Heldt, Scott

AU - Zhao, Meilan

AU - Schroeder, Jason P.

AU - Vellano, Christopher P.

AU - Cowan, D. Patrick

AU - Ramineni, Suneela

AU - Yates, Cindee K.

AU - Feng, Yue

AU - Smith, Yoland

AU - David Sweatt, J.

AU - Weinshenker, David

AU - Ressler, Kerry J.

AU - Dudek, Serena M.

AU - Hepler, John R.

PY - 2010/9/28

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