Ribonuclease 7, an antimicrobial peptide upregulated during infection, contributes to microbial defense of the human urinary tract

John David Spencer, Andrew L. Schwaderer, Huanyu Wang, Julianne Bartz, Jennifer Kline, Tad Eichler, Kristin R. Desouza, Sunder Sims-Lucas, Peter Baker, David Hains

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The mechanisms that maintain sterility in the urinary tract are incompletely understood; however, recent studies stress the importance of antimicrobial peptides in protecting the urinary tract from infection. Ribonuclease 7 (RNase 7), a potent antimicrobial peptide contributing to urinary tract sterility, is expressed by intercalated cells in the renal collecting tubules and is present in the urine at levels sufficient to kill bacteria at baseline. Here, we characterize the expression and function of RNase 7 in the human urinary tract during infection. Both quantitative real-time PCR and enzyme-linked immunosorbant assays demonstrated increases in RNASE7 expression in the kidney along with kidney and urinary RNase 7 peptide concentrations with infection. While immunostaining localized RNase 7 production to the intercalated cells of the collecting tubule during sterility, its expression during pyelonephritis was found to increase throughout the nephron but not in glomeruli or the interstitium. Recombinant RNase 7 exhibited antimicrobial activity against uropathogens at low micromolar concentrations by disrupting the microbial membrane as determined by atomic force microscopy. Thus, RNase 7 expression is increased in the urinary tract with infection and has antibacterial activity against uropathogens at micromolar concentrations.

Original languageEnglish (US)
Pages (from-to)615-625
Number of pages11
JournalKidney International
Volume83
Issue number4
DOIs
StatePublished - Jan 1 2013

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Urinary Tract
Peptides
Infection
Urinary Tract Infections
Infertility
Kidney
Atomic Force Microscopy
Pyelonephritis
Nephrons
Ribonuclease 7
Real-Time Polymerase Chain Reaction
Urine
Bacteria
Membranes
Enzymes

All Science Journal Classification (ASJC) codes

  • Nephrology

Cite this

Ribonuclease 7, an antimicrobial peptide upregulated during infection, contributes to microbial defense of the human urinary tract. / Spencer, John David; Schwaderer, Andrew L.; Wang, Huanyu; Bartz, Julianne; Kline, Jennifer; Eichler, Tad; Desouza, Kristin R.; Sims-Lucas, Sunder; Baker, Peter; Hains, David.

In: Kidney International, Vol. 83, No. 4, 01.01.2013, p. 615-625.

Research output: Contribution to journalArticle

Spencer, JD, Schwaderer, AL, Wang, H, Bartz, J, Kline, J, Eichler, T, Desouza, KR, Sims-Lucas, S, Baker, P & Hains, D 2013, 'Ribonuclease 7, an antimicrobial peptide upregulated during infection, contributes to microbial defense of the human urinary tract', Kidney International, vol. 83, no. 4, pp. 615-625. https://doi.org/10.1038/ki.2012.410
Spencer, John David ; Schwaderer, Andrew L. ; Wang, Huanyu ; Bartz, Julianne ; Kline, Jennifer ; Eichler, Tad ; Desouza, Kristin R. ; Sims-Lucas, Sunder ; Baker, Peter ; Hains, David. / Ribonuclease 7, an antimicrobial peptide upregulated during infection, contributes to microbial defense of the human urinary tract. In: Kidney International. 2013 ; Vol. 83, No. 4. pp. 615-625.
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