Ribonuclease 7 is a potent antimicrobial peptide within the human urinary tract

John D. Spencer, Andrew L. Schwaderer, Julianne D. Dirosario, Kirk M. McHugh, Glen McGillivary, Sheryl S. Justice, Ashley R. Carpenter, Peter B. Baker, Jürgen Harder, David Hains

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Although the urinary tract is constantly challenged by microbial invasion, it remains free from colonization. Although little is known about how the urinary tract maintains sterility, the presence of antimicrobial peptides (AMPs) in the urine suggests that they may play a role in its protection from infection. Ribonuclease 7 (RNase 7) is a potent AMP that was first identified in the skin. Here, we characterize the expression and relevance of RNase 7 in the human kidney and urinary tract. Using RNA isolated from healthy human tissue, we performed quantitative real-time PCR and found basal RNASE7 expression in kidney and bladder tissue. Immunohistochemical and immunofluorescent analysis localized RNase 7 to the urothelium of the bladder, ureter, and the intercalated cells of the collecting tubules. In control urine samples from healthy individuals, the concentration of RNase 7 was found to be in the low micromolar range; very abundant for an AMP. Antibacterial neutralization assays showed that urinary RNase 7 has potent antimicrobial properties against Gram-negative and Gram-positive uropathogenic bacteria. Thus, RNase 7 is expressed in the human kidney and urinary tract and it may have an important antimicrobial role in maintaining tract sterility.

Original languageEnglish (US)
Pages (from-to)174-180
Number of pages7
JournalKidney International
Volume80
Issue number2
DOIs
StatePublished - Jul 2 2011
Externally publishedYes

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Urinary Tract
Peptides
Kidney
Infertility
Urinary Bladder
Urine
Urothelium
Gram-Positive Bacteria
Ureter
Ribonuclease 7
Real-Time Polymerase Chain Reaction
RNA
Skin
Infection

All Science Journal Classification (ASJC) codes

  • Nephrology

Cite this

Spencer, J. D., Schwaderer, A. L., Dirosario, J. D., McHugh, K. M., McGillivary, G., Justice, S. S., ... Hains, D. (2011). Ribonuclease 7 is a potent antimicrobial peptide within the human urinary tract. Kidney International, 80(2), 174-180. https://doi.org/10.1038/ki.2011.109

Ribonuclease 7 is a potent antimicrobial peptide within the human urinary tract. / Spencer, John D.; Schwaderer, Andrew L.; Dirosario, Julianne D.; McHugh, Kirk M.; McGillivary, Glen; Justice, Sheryl S.; Carpenter, Ashley R.; Baker, Peter B.; Harder, Jürgen; Hains, David.

In: Kidney International, Vol. 80, No. 2, 02.07.2011, p. 174-180.

Research output: Contribution to journalArticle

Spencer, JD, Schwaderer, AL, Dirosario, JD, McHugh, KM, McGillivary, G, Justice, SS, Carpenter, AR, Baker, PB, Harder, J & Hains, D 2011, 'Ribonuclease 7 is a potent antimicrobial peptide within the human urinary tract', Kidney International, vol. 80, no. 2, pp. 174-180. https://doi.org/10.1038/ki.2011.109
Spencer JD, Schwaderer AL, Dirosario JD, McHugh KM, McGillivary G, Justice SS et al. Ribonuclease 7 is a potent antimicrobial peptide within the human urinary tract. Kidney International. 2011 Jul 2;80(2):174-180. https://doi.org/10.1038/ki.2011.109
Spencer, John D. ; Schwaderer, Andrew L. ; Dirosario, Julianne D. ; McHugh, Kirk M. ; McGillivary, Glen ; Justice, Sheryl S. ; Carpenter, Ashley R. ; Baker, Peter B. ; Harder, Jürgen ; Hains, David. / Ribonuclease 7 is a potent antimicrobial peptide within the human urinary tract. In: Kidney International. 2011 ; Vol. 80, No. 2. pp. 174-180.
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