Role of bacterial components in macrophage activation by the LAC and MW2 strains of community-associated, methicillin-resistant Staphylococcus aureus

Thomas Spentzas, Ravi Kudumula, Carlos Acuna, Ajay Talati, Kimberly C. Ingram, Fabio Savorgnan, Elizabeth A. Meals, B. Keith English

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We tested the contribution of four staphylococcal components - PSM-α, PSM-β, δ-toxin, and PVL - in triggering macrophage secretion of tumor necrosis factor (TNF) and interleukins 6 (IL-6) and 12 (IL-12) by two prominent, circulating strains of community-associated, methicillin-resistant Staphylococcus aureus (CA-MRSA): LAC, USA300; MW2, USA400. RAW 264.7 murine macrophages were stimulated with live, antibiotic-exposed bacteria, and cytokine secretion was quantitated in supernatants. Deletion of PSM-α expression in LAC led to >50% reduction in macrophage TNF and IL-6 secretion and a 20% reduction in IL-12 secretion, while PSM-α deletion in MW2 did not significantly reduce macrophage TNF secretion but resulted in a 15-20% reduction in IL-6 and IL-12 secretion. Deletion of δ-toxin in either strain led to more than 50% reduction in macrophage IL-6 secretion and smaller reductions in macrophage TNF and IL-12 secretion (8-25%). Our data implicate both PSM-α and δ-toxin in stimulating macrophage cytokine responses to CA-MRSA bacteria.

Original languageEnglish (US)
Pages (from-to)46-53
Number of pages8
JournalCellular Immunology
Volume269
Issue number1
DOIs
StatePublished - Apr 1 2011

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Macrophage Activation
Methicillin-Resistant Staphylococcus aureus
Interleukin-6
Macrophages
Interleukin-12
Tumor Necrosis Factor-alpha
Cytokines
Bacteria
Anti-Bacterial Agents

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Role of bacterial components in macrophage activation by the LAC and MW2 strains of community-associated, methicillin-resistant Staphylococcus aureus. / Spentzas, Thomas; Kudumula, Ravi; Acuna, Carlos; Talati, Ajay; Ingram, Kimberly C.; Savorgnan, Fabio; Meals, Elizabeth A.; English, B. Keith.

In: Cellular Immunology, Vol. 269, No. 1, 01.04.2011, p. 46-53.

Research output: Contribution to journalArticle

Spentzas, Thomas ; Kudumula, Ravi ; Acuna, Carlos ; Talati, Ajay ; Ingram, Kimberly C. ; Savorgnan, Fabio ; Meals, Elizabeth A. ; English, B. Keith. / Role of bacterial components in macrophage activation by the LAC and MW2 strains of community-associated, methicillin-resistant Staphylococcus aureus. In: Cellular Immunology. 2011 ; Vol. 269, No. 1. pp. 46-53.
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