Role of CCAAT enhancer-binding protein β in the thyroid hormone and cAMP induction of phosphoenolpyruvate carboxykinase gene transcription

Edwards Park, Shulan Song, Charles Vinson, William J. Roesler

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Transcription of the gene for phosphoenolpyruvate carboxykinase (PEPCK) is stimulated by thyroid hormone (T3) and cAMP. Two DNA elements in the PEPCK promoter are required for T3 responsiveness including a thyroid hormone response element and a binding site called P3(I) for the CCAAT enhancer-binding protein (C/EBP). Both the α and β isoforms of C/EBP are highly expressed in the liver. C/EBPα contributes to the liver-specific expression and cAMP responsiveness of the PEPCK gene. In this study, we examined the ability of C/EBPβ when bound to the P3(I) site to regulate PEPCK gene expression. We report that C/EBPβ can stimulate basal expression and participate in the induction of PEPCK gene transcription by T3 and cAMP. The cAMP-responsive element-binding protein and AP1 proteins that contribute to the induction by cAMP are not involved in the stimulation by T3. A small region of the transactivation domain of C/EBPβ is sufficient for the stimulation of basal expression and cAMP responsiveness. Our results suggest that C/EBPα and C/EBPβ are functionally interchangeable when bound to the P3(I) site of the PEPCK promoter.

Original languageEnglish (US)
Pages (from-to)211-217
Number of pages7
JournalJournal of Biological Chemistry
Volume274
Issue number1
DOIs
StatePublished - Jan 1 1999

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CCAAT-Enhancer-Binding Proteins
Phosphoenolpyruvate
Transcription
Thyroid Hormones
Genes
Liver
Response Elements
Triiodothyronine
Gene expression
Transcriptional Activation
Carrier Proteins
Protein Isoforms
Binding Sites
Gene Expression
DNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Role of CCAAT enhancer-binding protein β in the thyroid hormone and cAMP induction of phosphoenolpyruvate carboxykinase gene transcription. / Park, Edwards; Song, Shulan; Vinson, Charles; Roesler, William J.

In: Journal of Biological Chemistry, Vol. 274, No. 1, 01.01.1999, p. 211-217.

Research output: Contribution to journalArticle

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