Role of central serotonin systems in the stimulatory effects of ovarian hormones and naloxone on luteinizing hormone release in female rats

Mahlon Johnson, William R. Crowley, B. L. Carroll

Research output: Contribution to journalArticle

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Abstract

The present experiments tested whether serotonergic neurons that innervate discrete areas of the hypothalamus are involved in the stimulation of LH release by ovarian hormones or the opiate antagonist naloxone in female rats. Ovariectomized rats received oil vehicle, estradiol benzoate (EB) alone, or EB followed by progesterone (P) 2 days later. Serotonin (5-HT) activity was assessed from the accumulation observed after the administration of the monoamine oxidase inhibitor pargyline. Two days after EB treatment, LH concentrations were reduced in the morning and rose by later afternoon. Administration of P to EB-primed rats stimulated a LH surge. This latter treatment also enhanced pargyline-induced 5-HT accumulation, suggesting increased 5-HT activity, in the medial preoptic nucleus and interstitial nucleus of the stria terminalis, but reduced 5-HT accumulation, suggesting decreased 5-HT activity, in the ventromedial nucleus. 5-HT activity was unaffected after EB alone, either in the morning or afternoon, or by the administration of naloxone to EB-primed rats. Specific depletion of 5-HT in the medial preoptic/stria terminalis area, achieved by microinjection of the neurotoxic indoleamine 5,7-dihydroxytryptamine resulted in a blockade of the LH surge induced by EB plus P. The present results suggest that central 5-HT neurons innervating the preoptic area are involved in the LH surge induced by progesterone, but not in the increases in LH occurring after treatment with estradiol alone or after blockade of opiate receptors.

Original languageEnglish (US)
Pages (from-to)1180-1186
Number of pages7
JournalEndocrinology
Volume118
Issue number3
DOIs
StatePublished - Jan 1 1986

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Naloxone
Luteinizing Hormone
Serotonin
Hormones
Pargyline
Preoptic Area
Opiate Alkaloids
5,7-Dihydroxytryptamine
Serotonergic Neurons
Septal Nuclei
Monoamine Oxidase Inhibitors
Microinjections
Opioid Receptors
Hypothalamus
Progesterone
estradiol 3-benzoate
Estradiol
Oils
Therapeutics
Neurons

All Science Journal Classification (ASJC) codes

  • Endocrinology

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Role of central serotonin systems in the stimulatory effects of ovarian hormones and naloxone on luteinizing hormone release in female rats. / Johnson, Mahlon; Crowley, William R.; Carroll, B. L.

In: Endocrinology, Vol. 118, No. 3, 01.01.1986, p. 1180-1186.

Research output: Contribution to journalArticle

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