Role of cytochrome B5 in modulating peroxide-supported CYP3A4 activity

Evidence for a conformational transition and cytochrome P450 heterogeneity

Santosh Kumar, Dmitri R. Davydov, James R. Halpert

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The role of cytochrome b5 (b5) in the α-naphthoflavone (α-NF)-mediated inhibition of H2O 2-supported 7-benzyloxyquinoline (7-BQ) debenzylation by heterologously expressed and purified cytochrome P450 3A4 (CYP3A4) was studied. Although α-NF showed negligible effect in an NADPH-dependent reconstituted system, inhibition of 7-BQ oxidation was observed in the H2O 2 system. Analysis of the effect of various constituents of a standard reconstituted system on H2O2-supported activity showed that b5 alone resulted in a 2.5-fold increase in the k cat value and reversed the inhibitory effect of α-NF. In addition, titration with b5 suggested that only 65% of the CYP3A4 participated in the interaction with b5, consistent with cytochrome P450 (P450) heterogeneity. Study of the influence of b5 on the kinetics of H2O2-dependent destruction of the P450 heme moiety suggested two distinct conformers of CYP3A4 with different sensitivity to heme loss. In the absence of b5, 66% of the wild-type enzyme was bleached in the fast phase, whereas the addition of b5 decreased the fraction of the fast phase to 16%. Finally, to locate amino acid residues that might influence b5 action, several active site mutants were tested. Substitution of Ser-119, Ile-301, Ala-305, Ile-369, or Ala-370 with the larger Phe or Trp decreased or even abolished the activation by b5. Ser-119 is in the B′-C loop, a predicted b5-P450 interaction site, and Ile-301 and Ala-305 are closest to the heme. In conclusion, the interaction of b5 with P450 apparently leads to a conformational transition, which results in redistribution of the CYP3A4 pool.

Original languageEnglish (US)
Pages (from-to)1131-1136
Number of pages6
JournalDrug Metabolism and Disposition
Volume33
Issue number8
DOIs
StatePublished - Aug 1 2005

Fingerprint

Cytochromes b5
Cytochrome P-450 CYP3A
Peroxides
Cytochrome P-450 Enzyme System
Heme
NADP
Catalytic Domain
Cats
Amino Acids
Enzymes
7-benzyloxyquinoline

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

Role of cytochrome B5 in modulating peroxide-supported CYP3A4 activity : Evidence for a conformational transition and cytochrome P450 heterogeneity. / Kumar, Santosh; Davydov, Dmitri R.; Halpert, James R.

In: Drug Metabolism and Disposition, Vol. 33, No. 8, 01.08.2005, p. 1131-1136.

Research output: Contribution to journalArticle

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abstract = "The role of cytochrome b5 (b5) in the α-naphthoflavone (α-NF)-mediated inhibition of H2O 2-supported 7-benzyloxyquinoline (7-BQ) debenzylation by heterologously expressed and purified cytochrome P450 3A4 (CYP3A4) was studied. Although α-NF showed negligible effect in an NADPH-dependent reconstituted system, inhibition of 7-BQ oxidation was observed in the H2O 2 system. Analysis of the effect of various constituents of a standard reconstituted system on H2O2-supported activity showed that b5 alone resulted in a 2.5-fold increase in the k cat value and reversed the inhibitory effect of α-NF. In addition, titration with b5 suggested that only 65{\%} of the CYP3A4 participated in the interaction with b5, consistent with cytochrome P450 (P450) heterogeneity. Study of the influence of b5 on the kinetics of H2O2-dependent destruction of the P450 heme moiety suggested two distinct conformers of CYP3A4 with different sensitivity to heme loss. In the absence of b5, 66{\%} of the wild-type enzyme was bleached in the fast phase, whereas the addition of b5 decreased the fraction of the fast phase to 16{\%}. Finally, to locate amino acid residues that might influence b5 action, several active site mutants were tested. Substitution of Ser-119, Ile-301, Ala-305, Ile-369, or Ala-370 with the larger Phe or Trp decreased or even abolished the activation by b5. Ser-119 is in the B′-C loop, a predicted b5-P450 interaction site, and Ile-301 and Ala-305 are closest to the heme. In conclusion, the interaction of b5 with P450 apparently leads to a conformational transition, which results in redistribution of the CYP3A4 pool.",
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AB - The role of cytochrome b5 (b5) in the α-naphthoflavone (α-NF)-mediated inhibition of H2O 2-supported 7-benzyloxyquinoline (7-BQ) debenzylation by heterologously expressed and purified cytochrome P450 3A4 (CYP3A4) was studied. Although α-NF showed negligible effect in an NADPH-dependent reconstituted system, inhibition of 7-BQ oxidation was observed in the H2O 2 system. Analysis of the effect of various constituents of a standard reconstituted system on H2O2-supported activity showed that b5 alone resulted in a 2.5-fold increase in the k cat value and reversed the inhibitory effect of α-NF. In addition, titration with b5 suggested that only 65% of the CYP3A4 participated in the interaction with b5, consistent with cytochrome P450 (P450) heterogeneity. Study of the influence of b5 on the kinetics of H2O2-dependent destruction of the P450 heme moiety suggested two distinct conformers of CYP3A4 with different sensitivity to heme loss. In the absence of b5, 66% of the wild-type enzyme was bleached in the fast phase, whereas the addition of b5 decreased the fraction of the fast phase to 16%. Finally, to locate amino acid residues that might influence b5 action, several active site mutants were tested. Substitution of Ser-119, Ile-301, Ala-305, Ile-369, or Ala-370 with the larger Phe or Trp decreased or even abolished the activation by b5. Ser-119 is in the B′-C loop, a predicted b5-P450 interaction site, and Ile-301 and Ala-305 are closest to the heme. In conclusion, the interaction of b5 with P450 apparently leads to a conformational transition, which results in redistribution of the CYP3A4 pool.

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