Role of DNA-Protein Interactions in Bacteriophage ΦX174 DNA Injection

Leodevico L. Ilag, Jean K. Tuech, Laura Thoma, Roberta A. Sumrada, Nino L. Incardona

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Like most bacteriophages, ΦX174 transfers its DNA through the cell wall, leaving an empty capsid on the cell surface. The process begins with ejection of the genome at its host-receptor site. The rate of this event can be measured, so detailed structure/function analysis of the mechanism is possible now that an atomic structure of the ΦX174 protein shell has been obtained. Amino acid substitutions at two arginine residues near the DNA-binding pocket of F capsid protein decrease the eclipse rate, while deletion of 27 bases from the J-F non-coding region increase the rate. An alanine to serine change in the N-terminal region of the ΦX174 H "spike" protein has suppressor activity in that this mutation also increases the eclipse rate when the complete genome is present within both mutant and wild-type F capsids. These results suggest that a portion of H protein is inside the capsid, and disruption of DNA-protein interactions is involved in the ejection mechanism.

Original languageEnglish (US)
Pages (from-to)671-684
Number of pages14
JournalJournal of Molecular Biology
Volume229
Issue number3
DOIs
StatePublished - Feb 5 1993

Fingerprint

Bacteriophages
Capsid
Injections
DNA
Proteins
Genome
Capsid Proteins
Amino Acid Substitution
Alanine
Cell Wall
Serine
Arginine
Mutation

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Cite this

Ilag, L. L., Tuech, J. K., Thoma, L., Sumrada, R. A., & Incardona, N. L. (1993). Role of DNA-Protein Interactions in Bacteriophage ΦX174 DNA Injection. Journal of Molecular Biology, 229(3), 671-684. https://doi.org/10.1006/jmbi.1993.1071

Role of DNA-Protein Interactions in Bacteriophage ΦX174 DNA Injection. / Ilag, Leodevico L.; Tuech, Jean K.; Thoma, Laura; Sumrada, Roberta A.; Incardona, Nino L.

In: Journal of Molecular Biology, Vol. 229, No. 3, 05.02.1993, p. 671-684.

Research output: Contribution to journalArticle

Ilag, LL, Tuech, JK, Thoma, L, Sumrada, RA & Incardona, NL 1993, 'Role of DNA-Protein Interactions in Bacteriophage ΦX174 DNA Injection', Journal of Molecular Biology, vol. 229, no. 3, pp. 671-684. https://doi.org/10.1006/jmbi.1993.1071
Ilag, Leodevico L. ; Tuech, Jean K. ; Thoma, Laura ; Sumrada, Roberta A. ; Incardona, Nino L. / Role of DNA-Protein Interactions in Bacteriophage ΦX174 DNA Injection. In: Journal of Molecular Biology. 1993 ; Vol. 229, No. 3. pp. 671-684.
@article{8429e49117ab4707bdb283359d975d68,
title = "Role of DNA-Protein Interactions in Bacteriophage ΦX174 DNA Injection",
abstract = "Like most bacteriophages, ΦX174 transfers its DNA through the cell wall, leaving an empty capsid on the cell surface. The process begins with ejection of the genome at its host-receptor site. The rate of this event can be measured, so detailed structure/function analysis of the mechanism is possible now that an atomic structure of the ΦX174 protein shell has been obtained. Amino acid substitutions at two arginine residues near the DNA-binding pocket of F capsid protein decrease the eclipse rate, while deletion of 27 bases from the J-F non-coding region increase the rate. An alanine to serine change in the N-terminal region of the ΦX174 H {"}spike{"} protein has suppressor activity in that this mutation also increases the eclipse rate when the complete genome is present within both mutant and wild-type F capsids. These results suggest that a portion of H protein is inside the capsid, and disruption of DNA-protein interactions is involved in the ejection mechanism.",
author = "Ilag, {Leodevico L.} and Tuech, {Jean K.} and Laura Thoma and Sumrada, {Roberta A.} and Incardona, {Nino L.}",
year = "1993",
month = "2",
day = "5",
doi = "10.1006/jmbi.1993.1071",
language = "English (US)",
volume = "229",
pages = "671--684",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Role of DNA-Protein Interactions in Bacteriophage ΦX174 DNA Injection

AU - Ilag, Leodevico L.

AU - Tuech, Jean K.

AU - Thoma, Laura

AU - Sumrada, Roberta A.

AU - Incardona, Nino L.

PY - 1993/2/5

Y1 - 1993/2/5

N2 - Like most bacteriophages, ΦX174 transfers its DNA through the cell wall, leaving an empty capsid on the cell surface. The process begins with ejection of the genome at its host-receptor site. The rate of this event can be measured, so detailed structure/function analysis of the mechanism is possible now that an atomic structure of the ΦX174 protein shell has been obtained. Amino acid substitutions at two arginine residues near the DNA-binding pocket of F capsid protein decrease the eclipse rate, while deletion of 27 bases from the J-F non-coding region increase the rate. An alanine to serine change in the N-terminal region of the ΦX174 H "spike" protein has suppressor activity in that this mutation also increases the eclipse rate when the complete genome is present within both mutant and wild-type F capsids. These results suggest that a portion of H protein is inside the capsid, and disruption of DNA-protein interactions is involved in the ejection mechanism.

AB - Like most bacteriophages, ΦX174 transfers its DNA through the cell wall, leaving an empty capsid on the cell surface. The process begins with ejection of the genome at its host-receptor site. The rate of this event can be measured, so detailed structure/function analysis of the mechanism is possible now that an atomic structure of the ΦX174 protein shell has been obtained. Amino acid substitutions at two arginine residues near the DNA-binding pocket of F capsid protein decrease the eclipse rate, while deletion of 27 bases from the J-F non-coding region increase the rate. An alanine to serine change in the N-terminal region of the ΦX174 H "spike" protein has suppressor activity in that this mutation also increases the eclipse rate when the complete genome is present within both mutant and wild-type F capsids. These results suggest that a portion of H protein is inside the capsid, and disruption of DNA-protein interactions is involved in the ejection mechanism.

UR - http://www.scopus.com/inward/record.url?scp=0027407151&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027407151&partnerID=8YFLogxK

U2 - 10.1006/jmbi.1993.1071

DO - 10.1006/jmbi.1993.1071

M3 - Article

VL - 229

SP - 671

EP - 684

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 3

ER -