Role of Elovl4 protein in the biosynthesis of docosahexaenoic acid

Martin Paul Agbaga, Richard S. Brush, Nawajes Mandal, Michael H. Elliott, Muayyad R. Al-Ubaidi, Robert E. Anderson

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Citations (Scopus)

Abstract

The disk membranes of retinal photoreceptor outer segments and other neuronal and reproductive tissues are enriched in docosahexaenoic acid (DHA, 22:6n3), which is essential for their normal function and development. The fatty acid condensing enzyme Elongation of Very Long chain fatty acids-4 (ELOVL4) is highly expressed in retina photoreceptors as well as other tissues with high 22:6n3 content. Mutations in the ELOVL4 gene are associated with autosomal dominant Stargardt-like macular dystrophy (STGD3) and results in synthesis of a truncated protein that cannot be targeted to the endoplasmic reticulum (ER), the site of fatty acid biosynthesis. Considering the abundance and essential roles of 22:6n3 in ELOVL4-expressing tissues (except the skin), it was proposed that the ELOVL4 protein may be involved in 22:6n3 biosynthesis. We tested the hypothesis that the ELOVL4 protein is involved in 22:6n3 biosynthesis by selectively silencing expression of the protein in the cone photoreceptors derived cell line 661W and showed that the ELOVL4 protein is not involved in DHA biosynthesis from the short chain fatty acid precursors 18:3n3 and 22:5n3.

Original languageEnglish (US)
Title of host publicationRetinal Degenerative Diseases
Subtitle of host publicationLaboratory and Therapeutic Investigations
EditorsRobert Anderson, Nawajes Mandal, Joe Hollyfield, Matthew LaVail
Pages233-242
Number of pages10
DOIs
StatePublished - Dec 1 2010
Externally publishedYes

Publication series

NameAdvances in Experimental Medicine and Biology
Volume664
ISSN (Print)0065-2598

Fingerprint

Docosahexaenoic Acids
Biosynthesis
Protein Biosynthesis
Fatty Acids
Tissue
Proteins
Retinal Photoreceptor Cell Outer Segment
Retinal Cone Photoreceptor Cells
Volatile Fatty Acids
Endoplasmic Reticulum
Retina
Cones
Elongation
Skin
Genes
Cells
Membranes
Cell Line
Mutation
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Agbaga, M. P., Brush, R. S., Mandal, N., Elliott, M. H., Al-Ubaidi, M. R., & Anderson, R. E. (2010). Role of Elovl4 protein in the biosynthesis of docosahexaenoic acid. In R. Anderson, N. Mandal, J. Hollyfield, & M. LaVail (Eds.), Retinal Degenerative Diseases: Laboratory and Therapeutic Investigations (pp. 233-242). (Advances in Experimental Medicine and Biology; Vol. 664). https://doi.org/10.1007/978-1-4419-1399-9_27

Role of Elovl4 protein in the biosynthesis of docosahexaenoic acid. / Agbaga, Martin Paul; Brush, Richard S.; Mandal, Nawajes; Elliott, Michael H.; Al-Ubaidi, Muayyad R.; Anderson, Robert E.

Retinal Degenerative Diseases: Laboratory and Therapeutic Investigations. ed. / Robert Anderson; Nawajes Mandal; Joe Hollyfield; Matthew LaVail. 2010. p. 233-242 (Advances in Experimental Medicine and Biology; Vol. 664).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Agbaga, MP, Brush, RS, Mandal, N, Elliott, MH, Al-Ubaidi, MR & Anderson, RE 2010, Role of Elovl4 protein in the biosynthesis of docosahexaenoic acid. in R Anderson, N Mandal, J Hollyfield & M LaVail (eds), Retinal Degenerative Diseases: Laboratory and Therapeutic Investigations. Advances in Experimental Medicine and Biology, vol. 664, pp. 233-242. https://doi.org/10.1007/978-1-4419-1399-9_27
Agbaga MP, Brush RS, Mandal N, Elliott MH, Al-Ubaidi MR, Anderson RE. Role of Elovl4 protein in the biosynthesis of docosahexaenoic acid. In Anderson R, Mandal N, Hollyfield J, LaVail M, editors, Retinal Degenerative Diseases: Laboratory and Therapeutic Investigations. 2010. p. 233-242. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-1-4419-1399-9_27
Agbaga, Martin Paul ; Brush, Richard S. ; Mandal, Nawajes ; Elliott, Michael H. ; Al-Ubaidi, Muayyad R. ; Anderson, Robert E. / Role of Elovl4 protein in the biosynthesis of docosahexaenoic acid. Retinal Degenerative Diseases: Laboratory and Therapeutic Investigations. editor / Robert Anderson ; Nawajes Mandal ; Joe Hollyfield ; Matthew LaVail. 2010. pp. 233-242 (Advances in Experimental Medicine and Biology).
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