Role of fluoridated dentifrices in root caries formation in vitro

Franklin Garcia-Godoy, Catherine Flaitz, John Hicks

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Purpose: To evaluate in vitro root caries formation in human permanent teeth and to determine the effects of commercially available dentifrices containing different amounts of fluoride, while employing a well-tested artificial caries system using an acidified gel. Methods: Root surfaces from caries-free human permanent teeth (n=10) underwent debridement and fluoride-free prophylaxis. The tooth roots were sectioned into six portions, and acid-resistant varnish was placed with two sound root surface windows exposed on each tooth portion. Each portion from a single tooth was assigned to a treatment group: (1) No treatment control; (2) Denticious 5000 dentifrice (5,000 ppm F + xylitol); (3) PreviDent 5000 (5,000 ppm F); (4) AIM dentifrice (1,500 ppm F); (5) Listerine dentifrice (1,300 ppm F); and (6) Crest Regular Paste (1,500 ppm F). Tooth portions were treated with fresh dentifrice twice daily for 180 seconds, followed by fresh synthetic saliva rinsing over a 7-day period. Controls were exposed twice daily to fresh synthetic saliva rinsing over a 7-day period. In vitro root caries were created using an acidified gel (pH 4.25, 21 days). Longitudinal sections (three sections/tooth portion, 30 sections/group; 60 lesions/group) were evaluated for mean lesion depths (water imbibition, polarized light). Statistical analyses were performed using ANOVA and Duncan's Multiple Range test. Results: Mean lesion depths were 389 ± 43 tim for No treatment - control, 223 ± 33 pm for Denticious 5000 dentifrice, 242 ± 42 pm for Prevident 5000, 337 ± 29 pm for AIM dentifrice, 297 ± 37 pm for Listerine dentifrice, and 282 ± 34 pm for Crest Regular Paste dentifrice. All treatment groups had mean depths significantly less than the No treatment - control group (P< 0.05).Denticious 5000 and PreviDent 5000 had significantly reduced mean depth compared with the other dentifrice treatment groups (P< 0.05). CLINICAL SIGMFICANCE Fluoride-containing dentifrices provided significant reductions in mean in vitro lesion depths in root surfaces compared with control root surfaces not exposed to dentifrice treatment, considering the limitations of the in vitro artificial caries system. Denticious 5000 (5,000 ppm fluoride with xylitol) and PreviDent 5000 (5,000 ppm fluoride without xylitol) provided a greater degree of caries protection for root surfaces compared with dentifrices that contain 1,300 or 1,500 ppm fluoride. Dentifrices with higher fluoride content may be important in the prevention of caries in exposed root surfaces, especially in high caries-risk individuals.

Original languageEnglish (US)
Pages (from-to)23-28
Number of pages6
JournalAmerican journal of dentistry
Volume27
Issue number1
StatePublished - Jan 1 2014

Fingerprint

Root Caries
Dentifrices
Fluorides
Tooth
Xylitol
Ointments
Saliva
In Vitro Techniques
Gels
Tooth Root
Paint
Debridement

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Role of fluoridated dentifrices in root caries formation in vitro. / Garcia-Godoy, Franklin; Flaitz, Catherine; Hicks, John.

In: American journal of dentistry, Vol. 27, No. 1, 01.01.2014, p. 23-28.

Research output: Contribution to journalArticle

Garcia-Godoy, Franklin ; Flaitz, Catherine ; Hicks, John. / Role of fluoridated dentifrices in root caries formation in vitro. In: American journal of dentistry. 2014 ; Vol. 27, No. 1. pp. 23-28.
@article{609be6d11fb048daa360c4198ef5923c,
title = "Role of fluoridated dentifrices in root caries formation in vitro",
abstract = "Purpose: To evaluate in vitro root caries formation in human permanent teeth and to determine the effects of commercially available dentifrices containing different amounts of fluoride, while employing a well-tested artificial caries system using an acidified gel. Methods: Root surfaces from caries-free human permanent teeth (n=10) underwent debridement and fluoride-free prophylaxis. The tooth roots were sectioned into six portions, and acid-resistant varnish was placed with two sound root surface windows exposed on each tooth portion. Each portion from a single tooth was assigned to a treatment group: (1) No treatment control; (2) Denticious 5000 dentifrice (5,000 ppm F + xylitol); (3) PreviDent 5000 (5,000 ppm F); (4) AIM dentifrice (1,500 ppm F); (5) Listerine dentifrice (1,300 ppm F); and (6) Crest Regular Paste (1,500 ppm F). Tooth portions were treated with fresh dentifrice twice daily for 180 seconds, followed by fresh synthetic saliva rinsing over a 7-day period. Controls were exposed twice daily to fresh synthetic saliva rinsing over a 7-day period. In vitro root caries were created using an acidified gel (pH 4.25, 21 days). Longitudinal sections (three sections/tooth portion, 30 sections/group; 60 lesions/group) were evaluated for mean lesion depths (water imbibition, polarized light). Statistical analyses were performed using ANOVA and Duncan's Multiple Range test. Results: Mean lesion depths were 389 ± 43 tim for No treatment - control, 223 ± 33 pm for Denticious 5000 dentifrice, 242 ± 42 pm for Prevident 5000, 337 ± 29 pm for AIM dentifrice, 297 ± 37 pm for Listerine dentifrice, and 282 ± 34 pm for Crest Regular Paste dentifrice. All treatment groups had mean depths significantly less than the No treatment - control group (P< 0.05).Denticious 5000 and PreviDent 5000 had significantly reduced mean depth compared with the other dentifrice treatment groups (P< 0.05). CLINICAL SIGMFICANCE Fluoride-containing dentifrices provided significant reductions in mean in vitro lesion depths in root surfaces compared with control root surfaces not exposed to dentifrice treatment, considering the limitations of the in vitro artificial caries system. Denticious 5000 (5,000 ppm fluoride with xylitol) and PreviDent 5000 (5,000 ppm fluoride without xylitol) provided a greater degree of caries protection for root surfaces compared with dentifrices that contain 1,300 or 1,500 ppm fluoride. Dentifrices with higher fluoride content may be important in the prevention of caries in exposed root surfaces, especially in high caries-risk individuals.",
author = "Franklin Garcia-Godoy and Catherine Flaitz and John Hicks",
year = "2014",
month = "1",
day = "1",
language = "English (US)",
volume = "27",
pages = "23--28",
journal = "American Journal of Dentistry",
issn = "0894-8275",
publisher = "Mosher and Linder, Inc",
number = "1",

}

TY - JOUR

T1 - Role of fluoridated dentifrices in root caries formation in vitro

AU - Garcia-Godoy, Franklin

AU - Flaitz, Catherine

AU - Hicks, John

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Purpose: To evaluate in vitro root caries formation in human permanent teeth and to determine the effects of commercially available dentifrices containing different amounts of fluoride, while employing a well-tested artificial caries system using an acidified gel. Methods: Root surfaces from caries-free human permanent teeth (n=10) underwent debridement and fluoride-free prophylaxis. The tooth roots were sectioned into six portions, and acid-resistant varnish was placed with two sound root surface windows exposed on each tooth portion. Each portion from a single tooth was assigned to a treatment group: (1) No treatment control; (2) Denticious 5000 dentifrice (5,000 ppm F + xylitol); (3) PreviDent 5000 (5,000 ppm F); (4) AIM dentifrice (1,500 ppm F); (5) Listerine dentifrice (1,300 ppm F); and (6) Crest Regular Paste (1,500 ppm F). Tooth portions were treated with fresh dentifrice twice daily for 180 seconds, followed by fresh synthetic saliva rinsing over a 7-day period. Controls were exposed twice daily to fresh synthetic saliva rinsing over a 7-day period. In vitro root caries were created using an acidified gel (pH 4.25, 21 days). Longitudinal sections (three sections/tooth portion, 30 sections/group; 60 lesions/group) were evaluated for mean lesion depths (water imbibition, polarized light). Statistical analyses were performed using ANOVA and Duncan's Multiple Range test. Results: Mean lesion depths were 389 ± 43 tim for No treatment - control, 223 ± 33 pm for Denticious 5000 dentifrice, 242 ± 42 pm for Prevident 5000, 337 ± 29 pm for AIM dentifrice, 297 ± 37 pm for Listerine dentifrice, and 282 ± 34 pm for Crest Regular Paste dentifrice. All treatment groups had mean depths significantly less than the No treatment - control group (P< 0.05).Denticious 5000 and PreviDent 5000 had significantly reduced mean depth compared with the other dentifrice treatment groups (P< 0.05). CLINICAL SIGMFICANCE Fluoride-containing dentifrices provided significant reductions in mean in vitro lesion depths in root surfaces compared with control root surfaces not exposed to dentifrice treatment, considering the limitations of the in vitro artificial caries system. Denticious 5000 (5,000 ppm fluoride with xylitol) and PreviDent 5000 (5,000 ppm fluoride without xylitol) provided a greater degree of caries protection for root surfaces compared with dentifrices that contain 1,300 or 1,500 ppm fluoride. Dentifrices with higher fluoride content may be important in the prevention of caries in exposed root surfaces, especially in high caries-risk individuals.

AB - Purpose: To evaluate in vitro root caries formation in human permanent teeth and to determine the effects of commercially available dentifrices containing different amounts of fluoride, while employing a well-tested artificial caries system using an acidified gel. Methods: Root surfaces from caries-free human permanent teeth (n=10) underwent debridement and fluoride-free prophylaxis. The tooth roots were sectioned into six portions, and acid-resistant varnish was placed with two sound root surface windows exposed on each tooth portion. Each portion from a single tooth was assigned to a treatment group: (1) No treatment control; (2) Denticious 5000 dentifrice (5,000 ppm F + xylitol); (3) PreviDent 5000 (5,000 ppm F); (4) AIM dentifrice (1,500 ppm F); (5) Listerine dentifrice (1,300 ppm F); and (6) Crest Regular Paste (1,500 ppm F). Tooth portions were treated with fresh dentifrice twice daily for 180 seconds, followed by fresh synthetic saliva rinsing over a 7-day period. Controls were exposed twice daily to fresh synthetic saliva rinsing over a 7-day period. In vitro root caries were created using an acidified gel (pH 4.25, 21 days). Longitudinal sections (three sections/tooth portion, 30 sections/group; 60 lesions/group) were evaluated for mean lesion depths (water imbibition, polarized light). Statistical analyses were performed using ANOVA and Duncan's Multiple Range test. Results: Mean lesion depths were 389 ± 43 tim for No treatment - control, 223 ± 33 pm for Denticious 5000 dentifrice, 242 ± 42 pm for Prevident 5000, 337 ± 29 pm for AIM dentifrice, 297 ± 37 pm for Listerine dentifrice, and 282 ± 34 pm for Crest Regular Paste dentifrice. All treatment groups had mean depths significantly less than the No treatment - control group (P< 0.05).Denticious 5000 and PreviDent 5000 had significantly reduced mean depth compared with the other dentifrice treatment groups (P< 0.05). CLINICAL SIGMFICANCE Fluoride-containing dentifrices provided significant reductions in mean in vitro lesion depths in root surfaces compared with control root surfaces not exposed to dentifrice treatment, considering the limitations of the in vitro artificial caries system. Denticious 5000 (5,000 ppm fluoride with xylitol) and PreviDent 5000 (5,000 ppm fluoride without xylitol) provided a greater degree of caries protection for root surfaces compared with dentifrices that contain 1,300 or 1,500 ppm fluoride. Dentifrices with higher fluoride content may be important in the prevention of caries in exposed root surfaces, especially in high caries-risk individuals.

UR - http://www.scopus.com/inward/record.url?scp=84898909223&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84898909223&partnerID=8YFLogxK

M3 - Article

VL - 27

SP - 23

EP - 28

JO - American Journal of Dentistry

JF - American Journal of Dentistry

SN - 0894-8275

IS - 1

ER -