Role of follicular dendritic cells in the apoptosis of germinal center B cells

Dae Y. Hur, Dae J. Kim, Seonghan Kim, Young I. Kim, Daeho Cho, Dong S. Lee, Young Il Hwang, Ki Won Bae, Ka Y. Chang, Wang J. Lee

Research output: Contribution to journalArticle

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Abstract

Follicular dendritic cells (FDCs) provide the most obvious source of antigens, which are essential for the differentiation of GC B cells. It has been reported that most proliferating B cells in germinal centers undergo apoptosis. Quantitative histology shows macrophages with apoptotic debris throughout the germinal center, the highest frequency of these cells being found in the dense FDC network. Based on these findings, we hypothesized that FDC may be involved in an apoptotic pathway of the germinal center B cells. To prove this hypothesis, we performed double immunohistochemical analysis using anti-FDC mAb and peanut agglutinin (PNA), with their respective TUNEL kits. Collated data showed that a great proportion of the apoptotic cells, most of which were positive for PNA, were in close contact with FDC, which indicated an interaction between FDC and B cells in the apoptotic pathway. Further studies using double immunohistochemical staining and FACS analyses demonstrated the expression of Fas-ligand (FasL) in a subset of the FDC. These results suggest that FDC may play a role in the apoptosis of germinal center B cells via Fas-FasL interaction. (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)107-111
Number of pages5
JournalImmunology Letters
Volume72
Issue number2
DOIs
StatePublished - May 1 2000
Externally publishedYes

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Follicular Dendritic Cells
Germinal Center
B-Lymphocytes
Apoptosis
Peanut Agglutinin
Fas Ligand Protein
In Situ Nick-End Labeling
Histology
Macrophages
Staining and Labeling
Antigens

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Hur, D. Y., Kim, D. J., Kim, S., Kim, Y. I., Cho, D., Lee, D. S., ... Lee, W. J. (2000). Role of follicular dendritic cells in the apoptosis of germinal center B cells. Immunology Letters, 72(2), 107-111. https://doi.org/10.1016/S0165-2478(00)00166-8

Role of follicular dendritic cells in the apoptosis of germinal center B cells. / Hur, Dae Y.; Kim, Dae J.; Kim, Seonghan; Kim, Young I.; Cho, Daeho; Lee, Dong S.; Hwang, Young Il; Bae, Ki Won; Chang, Ka Y.; Lee, Wang J.

In: Immunology Letters, Vol. 72, No. 2, 01.05.2000, p. 107-111.

Research output: Contribution to journalArticle

Hur, DY, Kim, DJ, Kim, S, Kim, YI, Cho, D, Lee, DS, Hwang, YI, Bae, KW, Chang, KY & Lee, WJ 2000, 'Role of follicular dendritic cells in the apoptosis of germinal center B cells', Immunology Letters, vol. 72, no. 2, pp. 107-111. https://doi.org/10.1016/S0165-2478(00)00166-8
Hur, Dae Y. ; Kim, Dae J. ; Kim, Seonghan ; Kim, Young I. ; Cho, Daeho ; Lee, Dong S. ; Hwang, Young Il ; Bae, Ki Won ; Chang, Ka Y. ; Lee, Wang J. / Role of follicular dendritic cells in the apoptosis of germinal center B cells. In: Immunology Letters. 2000 ; Vol. 72, No. 2. pp. 107-111.
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