Role of hydroperoxyeicosatetraenoic acids in oxidative stress-induced activating protein 1 (Ap-1) activity

Rao Gadiparthi, Wayne C. Glasgow, Thomas E. Eling, Marschall S. Runge

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

We have previously reported that hydrogen peroxide, an active oxygen species and a cellular oxidant, induces c-Fos and c-Jun mRNA expression and DNA synthesis in vascular smooth muscle cells and that these events require arachidonic acid release and metabolism through the lipoxygenase pathway. Here we have identified the eicosanoids that mediate the hydrogen peroxide- induced growth-related events in these cells. Hydrogen peroxide stimulated the production of 12- and 15-hydroperoxyeicosatetraenoic acids in vascular smooth muscle cells. Both 12- and 15-hydroperoxyeicosatetraenoic acids induced the expression of c-Fos and c-Jun protein and increased activating protein 1 (AP-1) activity, as measured by AP-1-DNA binding and AP-1-dependent human collagenase promoter-driven chloramphenicol acetyltransferase reporter gene transcription. Hydrogen peroxide and arachidonic acid also induced the expression of c-Fos and c-Jun protein and AP-1 activity. Nordihydroguaiaretic acid, an inhibitor of the lipoxygenase pathway, significantly inhibited both hydrogen peroxide and arachidonic acid-stimulated c-Fos and c-Jun protein expression and AP-1 activity. Together, these findings suggest that hydrogen peroxide induces the production of eicosanoids and that the eicosanoids are potential mediators of the oxidative stress-stimulated growth-related events in vascular smooth muscle cells.

Original languageEnglish (US)
Pages (from-to)27760-27764
Number of pages5
JournalJournal of Biological Chemistry
Volume271
Issue number44
DOIs
StatePublished - Nov 13 1996
Externally publishedYes

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Oxidative stress
Hydrogen Peroxide
Oxidative Stress
Proto-Oncogene Proteins c-jun
Acids
Eicosanoids
Vascular Smooth Muscle
Arachidonic Acid
Smooth Muscle Myocytes
Muscle
Proteins
Cells
Masoprocol
Lipoxygenase Inhibitors
Chloramphenicol O-Acetyltransferase
Lipoxygenase
DNA
DNA-Binding Proteins
Collagenases
Transcription

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Role of hydroperoxyeicosatetraenoic acids in oxidative stress-induced activating protein 1 (Ap-1) activity. / Gadiparthi, Rao; Glasgow, Wayne C.; Eling, Thomas E.; Runge, Marschall S.

In: Journal of Biological Chemistry, Vol. 271, No. 44, 13.11.1996, p. 27760-27764.

Research output: Contribution to journalArticle

Gadiparthi, Rao ; Glasgow, Wayne C. ; Eling, Thomas E. ; Runge, Marschall S. / Role of hydroperoxyeicosatetraenoic acids in oxidative stress-induced activating protein 1 (Ap-1) activity. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 44. pp. 27760-27764.
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