Role of neuroexcitation in development of blood-brain barrier and oedematous changes following cerebral ischaemia and traumatic brain injury.

N. Saito, C. Chang, K. Kawai, F. Joó, Thaddeus Nowak, G. Mies, J. Ikeda, G. Nagashima, C. Ruetzler, J. Lohr

Research output: Contribution to journalArticle

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Abstract

Potential involvement of neuroexcitatory mechanisms was studied in: 1) repetitive forebrain ischaemia in gerbils, 2) global cerebral ischaemia in rats and 3) cryogenic injury to the cerebral cortex in rats and gerbils. Uptake of 45Ca was used as a marker of injury, whereas ultrastructural localization of calcium was assessed with an oxalate-pyroantimonate method. The blood-brain barrier was evaluated with immunostaining for serum albumin. Changes in extracellular glutamate were estimated by microdialysis and an enzymatic cycling assay. Changes in water content were assessed by specific gravity measurements. Repetitive ischaemia of 3 x 5 min carotid occlusions produced a cumulative effect with regard to development of oedema and neuronal injury. This was associated with several-fold increments in glutamate release after repeated insults, whereas there was no apparent correlation with energy metabolism disturbances. Other studies revealed in all models a development of secondary foci distant to the primary impact of ischaemia or cold lesions, which were characterized by calcium accumulation in swollen dendrites, chronic neuronal changes and intraneuronal uptake of serum proteins, all of these changes being potentially compatible with involvement of neuroexcitatory mechanisms.

Original languageEnglish (US)
Pages (from-to)186-188
Number of pages3
JournalActa neurochirurgica. Supplementum
Volume51
StatePublished - Jan 1 1990
Externally publishedYes

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Brain Ischemia
Blood-Brain Barrier
Gerbillinae
Glutamic Acid
Wounds and Injuries
Ischemia
Calcium
Cold Ischemia
Specific Gravity
Oxalates
Microdialysis
Enzyme Assays
Prosencephalon
Dendrites
Serum Albumin
Cerebral Cortex
Energy Metabolism
Blood Proteins
Edema
Water

All Science Journal Classification (ASJC) codes

  • Surgery
  • Clinical Neurology

Cite this

Role of neuroexcitation in development of blood-brain barrier and oedematous changes following cerebral ischaemia and traumatic brain injury. / Saito, N.; Chang, C.; Kawai, K.; Joó, F.; Nowak, Thaddeus; Mies, G.; Ikeda, J.; Nagashima, G.; Ruetzler, C.; Lohr, J.

In: Acta neurochirurgica. Supplementum, Vol. 51, 01.01.1990, p. 186-188.

Research output: Contribution to journalArticle

Saito, N, Chang, C, Kawai, K, Joó, F, Nowak, T, Mies, G, Ikeda, J, Nagashima, G, Ruetzler, C & Lohr, J 1990, 'Role of neuroexcitation in development of blood-brain barrier and oedematous changes following cerebral ischaemia and traumatic brain injury.', Acta neurochirurgica. Supplementum, vol. 51, pp. 186-188.
Saito, N. ; Chang, C. ; Kawai, K. ; Joó, F. ; Nowak, Thaddeus ; Mies, G. ; Ikeda, J. ; Nagashima, G. ; Ruetzler, C. ; Lohr, J. / Role of neuroexcitation in development of blood-brain barrier and oedematous changes following cerebral ischaemia and traumatic brain injury. In: Acta neurochirurgica. Supplementum. 1990 ; Vol. 51. pp. 186-188.
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