Role of nuclear factor-κB in the antiviral action of interferon and interferon-regulated gene expression

Lawrence Pfeffer, Jong Gwan Kim, Susan R. Pfeffer, Dennis J. Carrigan, Darren P. Baker, Lai Weill, Ramin Homayouni

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Interferons (IFNs) play critical roles in host defense by modulating the expression of various genes via tyrosine phosphorylation of STAT transcription factors. IFN-α/β activates another important transcription factor, nuclear factor-κB (NF-κB), but its role in IFN-mediated activity is poorly understood. Sensitivity to the antiviral and gene-inducing effects of IFN was examined in normal fibroblasts and in NF-κB knockout fibroblasts from p50- and p65-null mice. Antiviral assays demonstrated that NF-κB knockout fibroblasts were sensitized to the antiviral action of IFN. Moreover, analysis of IFN-stimulated gene expression by real-time PCR demonstrated selective effects of NF-κB on gene expression. Our results demonstrate that a subset of IFN-stimulated genes is regulated through an NF-κB-dependent pathway and that NF-κB may regulate the sensitivity of cells to IFN-mediated antiviral activity.

Original languageEnglish (US)
Pages (from-to)31304-31311
Number of pages8
JournalJournal of Biological Chemistry
Volume279
Issue number30
DOIs
StatePublished - Jul 23 2004

Fingerprint

Gene expression
Interferons
Antiviral Agents
Gene Expression
Fibroblasts
Genes
STAT Transcription Factors
Phosphorylation
Tyrosine
Real-Time Polymerase Chain Reaction
Assays
Transcription Factors

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Role of nuclear factor-κB in the antiviral action of interferon and interferon-regulated gene expression. / Pfeffer, Lawrence; Kim, Jong Gwan; Pfeffer, Susan R.; Carrigan, Dennis J.; Baker, Darren P.; Weill, Lai; Homayouni, Ramin.

In: Journal of Biological Chemistry, Vol. 279, No. 30, 23.07.2004, p. 31304-31311.

Research output: Contribution to journalArticle

Pfeffer, Lawrence ; Kim, Jong Gwan ; Pfeffer, Susan R. ; Carrigan, Dennis J. ; Baker, Darren P. ; Weill, Lai ; Homayouni, Ramin. / Role of nuclear factor-κB in the antiviral action of interferon and interferon-regulated gene expression. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 30. pp. 31304-31311.
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