Role of platelet-activating factor in functional alterations induced by xenoreactive antibodies in porcine endothelial cells

Luigi Biancone, Vincenzo Cantaluppi, Giuseppe Segoloni, Mariarosaria Boccellino, Lorenzo Del Sorbo, Pier Giulio Conaldi, Larry W. Tjoelker, Shoici Maruyama, Edward Cantu, David Stern, Giuseppe Andres, Giovanni Camussi

Research output: Contribution to journalArticle

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Abstract

Background. Platelet-activating factor (PAF) is a phospholipid mediator of inflammation which has been implicated in rejection. The interaction of anti-α-galactosyl natural antibodies (anti-α gal Abs) with endothelial cells is the initial step for the development of xenograft rejection. In our study, we stimulated porcine aorthic endothelial cells (PAEC) with anti-α gal IgG to investigate the synthesis of PAF from PAEC and its biological consequences. Methods and Results. PAF was extracted and chromatografically purified from cultured PAEC stimulated with baboon anti-α gal Abs. The Abs induced a dose-dependent synthesis of PAF peaking after 39 min of incubation, and decreasing thereafter. Concomitant cell shape change, motility, and cytoskeleton redistribution were observed. These events were prevented by addition of a panel of PAF-receptor antagonists. An SV40 T-large antigen-immortalized PAEC line was engineered to express PAF acetyl-hydrolase (PAF-AH) cDNA, the major PAF-inactivating enzyme. These transfected cells exposed to anti-α gal Abs showed reduced cell contraction and motility compared with empty vector-transfected cells. Moreover, in PAEC stimulated with anti-α gal Abs, the synthesis of PAF promoted the adhesion of a monocytic cell line as shown by the inhibitory effect of PAF-receptor antagonists and of PAF-AH expression. Finally, studies on cell monolayer demonstrated an enhanced permeability 48 hr after exposure to anti-α gal Abs, and this increase was prevented by PAF-inactivation and by PAF-receptor blockade. Conclusions. These results demonstrate that on stimulation with anti-α gal Abs, PAEC synthetize PAF which can contribute to several vascular events involved in xenograft rejection.

Original languageEnglish (US)
Pages (from-to)1198-1205
Number of pages8
JournalTransplantation
Volume70
Issue number8
DOIs
StatePublished - Oct 27 2000
Externally publishedYes

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Platelet Activating Factor
Swine
Endothelial Cells
Antibodies
Heterografts
1-Alkyl-2-acetylglycerophosphocholine Esterase
Polyomavirus Transforming Antigens
Cell Line
Inflammation Mediators
Cell Shape
Papio
Population Growth
Cytoskeleton
Cell Movement
Blood Vessels
Permeability
Phospholipids
Complementary DNA
Enzymes

All Science Journal Classification (ASJC) codes

  • Transplantation

Cite this

Biancone, L., Cantaluppi, V., Segoloni, G., Boccellino, M., Del Sorbo, L., Conaldi, P. G., ... Camussi, G. (2000). Role of platelet-activating factor in functional alterations induced by xenoreactive antibodies in porcine endothelial cells. Transplantation, 70(8), 1198-1205. https://doi.org/10.1097/00007890-200010270-00013

Role of platelet-activating factor in functional alterations induced by xenoreactive antibodies in porcine endothelial cells. / Biancone, Luigi; Cantaluppi, Vincenzo; Segoloni, Giuseppe; Boccellino, Mariarosaria; Del Sorbo, Lorenzo; Conaldi, Pier Giulio; Tjoelker, Larry W.; Maruyama, Shoici; Cantu, Edward; Stern, David; Andres, Giuseppe; Camussi, Giovanni.

In: Transplantation, Vol. 70, No. 8, 27.10.2000, p. 1198-1205.

Research output: Contribution to journalArticle

Biancone, L, Cantaluppi, V, Segoloni, G, Boccellino, M, Del Sorbo, L, Conaldi, PG, Tjoelker, LW, Maruyama, S, Cantu, E, Stern, D, Andres, G & Camussi, G 2000, 'Role of platelet-activating factor in functional alterations induced by xenoreactive antibodies in porcine endothelial cells', Transplantation, vol. 70, no. 8, pp. 1198-1205. https://doi.org/10.1097/00007890-200010270-00013
Biancone, Luigi ; Cantaluppi, Vincenzo ; Segoloni, Giuseppe ; Boccellino, Mariarosaria ; Del Sorbo, Lorenzo ; Conaldi, Pier Giulio ; Tjoelker, Larry W. ; Maruyama, Shoici ; Cantu, Edward ; Stern, David ; Andres, Giuseppe ; Camussi, Giovanni. / Role of platelet-activating factor in functional alterations induced by xenoreactive antibodies in porcine endothelial cells. In: Transplantation. 2000 ; Vol. 70, No. 8. pp. 1198-1205.
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abstract = "Background. Platelet-activating factor (PAF) is a phospholipid mediator of inflammation which has been implicated in rejection. The interaction of anti-α-galactosyl natural antibodies (anti-α gal Abs) with endothelial cells is the initial step for the development of xenograft rejection. In our study, we stimulated porcine aorthic endothelial cells (PAEC) with anti-α gal IgG to investigate the synthesis of PAF from PAEC and its biological consequences. Methods and Results. PAF was extracted and chromatografically purified from cultured PAEC stimulated with baboon anti-α gal Abs. The Abs induced a dose-dependent synthesis of PAF peaking after 39 min of incubation, and decreasing thereafter. Concomitant cell shape change, motility, and cytoskeleton redistribution were observed. These events were prevented by addition of a panel of PAF-receptor antagonists. An SV40 T-large antigen-immortalized PAEC line was engineered to express PAF acetyl-hydrolase (PAF-AH) cDNA, the major PAF-inactivating enzyme. These transfected cells exposed to anti-α gal Abs showed reduced cell contraction and motility compared with empty vector-transfected cells. Moreover, in PAEC stimulated with anti-α gal Abs, the synthesis of PAF promoted the adhesion of a monocytic cell line as shown by the inhibitory effect of PAF-receptor antagonists and of PAF-AH expression. Finally, studies on cell monolayer demonstrated an enhanced permeability 48 hr after exposure to anti-α gal Abs, and this increase was prevented by PAF-inactivation and by PAF-receptor blockade. Conclusions. These results demonstrate that on stimulation with anti-α gal Abs, PAEC synthetize PAF which can contribute to several vascular events involved in xenograft rejection.",
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T1 - Role of platelet-activating factor in functional alterations induced by xenoreactive antibodies in porcine endothelial cells

AU - Biancone, Luigi

AU - Cantaluppi, Vincenzo

AU - Segoloni, Giuseppe

AU - Boccellino, Mariarosaria

AU - Del Sorbo, Lorenzo

AU - Conaldi, Pier Giulio

AU - Tjoelker, Larry W.

AU - Maruyama, Shoici

AU - Cantu, Edward

AU - Stern, David

AU - Andres, Giuseppe

AU - Camussi, Giovanni

PY - 2000/10/27

Y1 - 2000/10/27

N2 - Background. Platelet-activating factor (PAF) is a phospholipid mediator of inflammation which has been implicated in rejection. The interaction of anti-α-galactosyl natural antibodies (anti-α gal Abs) with endothelial cells is the initial step for the development of xenograft rejection. In our study, we stimulated porcine aorthic endothelial cells (PAEC) with anti-α gal IgG to investigate the synthesis of PAF from PAEC and its biological consequences. Methods and Results. PAF was extracted and chromatografically purified from cultured PAEC stimulated with baboon anti-α gal Abs. The Abs induced a dose-dependent synthesis of PAF peaking after 39 min of incubation, and decreasing thereafter. Concomitant cell shape change, motility, and cytoskeleton redistribution were observed. These events were prevented by addition of a panel of PAF-receptor antagonists. An SV40 T-large antigen-immortalized PAEC line was engineered to express PAF acetyl-hydrolase (PAF-AH) cDNA, the major PAF-inactivating enzyme. These transfected cells exposed to anti-α gal Abs showed reduced cell contraction and motility compared with empty vector-transfected cells. Moreover, in PAEC stimulated with anti-α gal Abs, the synthesis of PAF promoted the adhesion of a monocytic cell line as shown by the inhibitory effect of PAF-receptor antagonists and of PAF-AH expression. Finally, studies on cell monolayer demonstrated an enhanced permeability 48 hr after exposure to anti-α gal Abs, and this increase was prevented by PAF-inactivation and by PAF-receptor blockade. Conclusions. These results demonstrate that on stimulation with anti-α gal Abs, PAEC synthetize PAF which can contribute to several vascular events involved in xenograft rejection.

AB - Background. Platelet-activating factor (PAF) is a phospholipid mediator of inflammation which has been implicated in rejection. The interaction of anti-α-galactosyl natural antibodies (anti-α gal Abs) with endothelial cells is the initial step for the development of xenograft rejection. In our study, we stimulated porcine aorthic endothelial cells (PAEC) with anti-α gal IgG to investigate the synthesis of PAF from PAEC and its biological consequences. Methods and Results. PAF was extracted and chromatografically purified from cultured PAEC stimulated with baboon anti-α gal Abs. The Abs induced a dose-dependent synthesis of PAF peaking after 39 min of incubation, and decreasing thereafter. Concomitant cell shape change, motility, and cytoskeleton redistribution were observed. These events were prevented by addition of a panel of PAF-receptor antagonists. An SV40 T-large antigen-immortalized PAEC line was engineered to express PAF acetyl-hydrolase (PAF-AH) cDNA, the major PAF-inactivating enzyme. These transfected cells exposed to anti-α gal Abs showed reduced cell contraction and motility compared with empty vector-transfected cells. Moreover, in PAEC stimulated with anti-α gal Abs, the synthesis of PAF promoted the adhesion of a monocytic cell line as shown by the inhibitory effect of PAF-receptor antagonists and of PAF-AH expression. Finally, studies on cell monolayer demonstrated an enhanced permeability 48 hr after exposure to anti-α gal Abs, and this increase was prevented by PAF-inactivation and by PAF-receptor blockade. Conclusions. These results demonstrate that on stimulation with anti-α gal Abs, PAEC synthetize PAF which can contribute to several vascular events involved in xenograft rejection.

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