Role of the Mga regulon in the resistance of M type 4 Streptococcus pyogenes to phagocytosis

Harry Courtney, David L. Hasty, James Dale

Research output: Contribution to journalArticle

Abstract

M type 4 Streptococcus pyogenes is a frequent cause of infections, yet little is known concerning its anti-phagocytic factors. We investigated the role of the Mga regulon on growth in blood by inactivating individual genes within this regulon. Ablation of mrp4, emm4, or sof4 reduced streptococcal growth in human blood. Inactivation of enn4 had little impact on streptococcal growth in blood. Thus, expression of mrp, emm, and sof were required for optimal resistance to phagocytosis of M type 4 S. pyogenes. M proteins from class II serotypes contribute to resistance to phagocytosis by binding C4BP, a regulator of complement activation. Our data indicate that there is an additional pathway for resistance in these streptococci that is mediated by the binding of fibrinogen. Fibrinogen was required for growth of M type 4 S. pyogenes in blood and Mrp4 was the major fibrinogen-binding protein on these bacteria. Fibrinogen-Mrp interactions prevented activation of the classical pathway, thereby contributing to the ability of these streptococci to evade phagocytosis.

Original languageEnglish (US)
Pages (from-to)195-198
Number of pages4
JournalInternational Congress Series
Volume1289
DOIs
StatePublished - Apr 1 2006

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Regulon
Streptococcus pyogenes
Phagocytosis
Fibrinogen
Growth
Streptococcus
Complement Activation
Carrier Proteins
Bacteria
Infection
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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Role of the Mga regulon in the resistance of M type 4 Streptococcus pyogenes to phagocytosis. / Courtney, Harry; Hasty, David L.; Dale, James.

In: International Congress Series, Vol. 1289, 01.04.2006, p. 195-198.

Research output: Contribution to journalArticle

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