S-adenosylmethionine (SAMe) protects against acute alcohol induced hepatotoxicity in mice

Zhenyuan Song, Zhanxiang Zhou, Theresa Chen, Daniell Hill, Yujian Kang, Shirish Barve, Craig McClain

Research output: Contribution to journalArticle

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Abstract

Although S-Adenosylmethionine (SAMe) has beneficial effects in many hepatic disorders, the effects of SAMe on acute alcohol-induced liver injury are unknown. In the present study, we investigated effects of SAMe on liver injury in mice induced by acute alcohol administration. Male C57BL/6 mice received ethanol (5 g/kg BW) by gavage every 12 hrs for a total of 3 doses. SAMe (5 mg/kg BW) was administrated i.p. once a day for three days before ethanol administration. Subsequent serum ALT level, hepatic lipid peroxidation, enzymatic activity of CYP2E1 and hepatic mitochondrial glutathione levels were measured colorimetrically. Intracellular SAMe concentration was measured by high-performance liquid chromatography (HPLC). Histopathological changes were assessed by H&E staining. Our results showed that acute ethanol administration caused prominent microvesicular steatosis with mild necrosis and an elevation of serum ALT activity. SAMe treatment significantly attenuated the liver injury. In association with the hepatocyte injury, acute alcohol administration induced significant decreases in both hepatic SAMe and mitochondrial GSH levels along with enhanced lipid peroxidation. SAMe treatment attenuated hepatic SAMe and mitochondrial GSH depletion and lipid peroxidation following acute alcohol exposure. These results demonstrate that SAMe protects against the liver injury and attenuates the mitochondrial GSH depletion caused by acute alcohol administration. SAMe may prove to be an effective therapeutic agent in many toxin-induced liver injuries including those induced by alcohol.

Original languageEnglish (US)
Pages (from-to)591-597
Number of pages7
JournalJournal of Nutritional Biochemistry
Volume14
Issue number10
DOIs
StatePublished - Oct 1 2003

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S-Adenosylmethionine
Alcohols
Liver
Wounds and Injuries
Lipid Peroxidation
Ethanol
Lipids
Cytochrome P-450 CYP2E1
High performance liquid chromatography
Serum
Inbred C57BL Mouse
Glutathione
Hepatocytes
Necrosis
High Pressure Liquid Chromatography
Association reactions

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

S-adenosylmethionine (SAMe) protects against acute alcohol induced hepatotoxicity in mice. / Song, Zhenyuan; Zhou, Zhanxiang; Chen, Theresa; Hill, Daniell; Kang, Yujian; Barve, Shirish; McClain, Craig.

In: Journal of Nutritional Biochemistry, Vol. 14, No. 10, 01.10.2003, p. 591-597.

Research output: Contribution to journalArticle

Song, Zhenyuan ; Zhou, Zhanxiang ; Chen, Theresa ; Hill, Daniell ; Kang, Yujian ; Barve, Shirish ; McClain, Craig. / S-adenosylmethionine (SAMe) protects against acute alcohol induced hepatotoxicity in mice. In: Journal of Nutritional Biochemistry. 2003 ; Vol. 14, No. 10. pp. 591-597.
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